The yeast nuclear gene suv3 affecting mitochondrial post-transcriptional processes encodes a putative ATP-dependent RNA helicase - PubMed (original) (raw)

Comparative Study

The yeast nuclear gene suv3 affecting mitochondrial post-transcriptional processes encodes a putative ATP-dependent RNA helicase

P P Stepien et al. Proc Natl Acad Sci U S A. 1992.

Abstract

Mitochondrial gene expression is controlled largely through the action of products of the nuclear genome. The yeast nuclear gene suv3 has been implicated in a variety of mitochondrial posttranscriptional processes and in translation and, thus, represents a key control element in nuclear-mitochondrial interactions. We have exploited a property of a mutant allele of suv3, SUV3-1, that causes, among other effects, a massive increase in the abundance of excised group I introns to clone the wild-type gene by a strategy of colony Northern hybridization. We have determined that the 84-kDa deduced protein product of the suv3 gene, which maps to chromosome XVI, has a typical mitochondrial targeting presequence and additional sequence motifs that suggest that it belongs to a family of ATP-dependent RNA helicases, enzymes whose importance in post-transcriptional and translational events has recently become apparent. We have identified the SUV3-1 mutation as a G----T transversion that creates a Val----Leu substitution in a 10-amino acid block that is highly conserved among ATP-dependent RNA helicases. We discuss some implications of this mutation on the effects of the SUV3-1 allele on mitochondrial RNA metabolism.

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References

    1. Curr Genet. 1988 Jun;13(6):471-8 - PubMed
    1. Mol Cell Biol. 1989 Apr;9(4):1507-12 - PubMed
    1. Nature. 1988 May 5;333(6168):22-3 - PubMed
    1. Mol Gen Genet. 1971;111(3):209-23 - PubMed
    1. J Biol Chem. 1984 May 10;259(9):6019-27 - PubMed

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