Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells - PubMed (original) (raw)
. 2004 Mar 19;279(12):11639-48.
doi: 10.1074/jbc.M312895200. Epub 2003 Dec 17.
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- PMID: 14684736
- DOI: 10.1074/jbc.M312895200
Free article
Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells
Lianjun Yang et al. J Biol Chem. 2004.
Free article
Retraction in
- Withdrawal: Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells.
Yang L, Li N, Wang C, Yu Y, Yuan L, Zhang M, Cao X. Yang L, et al. J Biol Chem. 2021 Jan-Jun;296:100762. doi: 10.1016/j.jbc.2021.100762. Epub 2021 May 23. J Biol Chem. 2021. PMID: 34237909 Free PMC article. No abstract available.
Expression of concern in
- Expression of Concern: Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells.
[No authors listed] [No authors listed] J Biol Chem. 2020 Jun 26;295(26):8875. doi: 10.1074/jbc.EC120.014562. J Biol Chem. 2020. PMID: 32591451 Free PMC article. No abstract available.
Abstract
We report the cloning and functional characterization of human cyclin L2, a novel member of the cyclin family. Human cyclin L2 shares significant homology to cyclin L1, K, T1, T2, and C, which are involved in transcriptional regulation via phosphorylation of the C-terminal domain of RNA polymerase II. The cyclin L2 protein contains an N-terminal "cyclin box" and C-terminal dipeptide repeats of alternating arginines and serines, a hallmark of the SR family of splicing factors. A new isoform and the mouse homologue of human cyclin L2 have also been cloned in this study. Human cyclin L2 is expressed ubiquitously in normal human tissues and tumor cells. We show here that cyclin L2 co-localizes with splicing factors SC-35 and 9G8 within nuclear speckles and that it associates with hyperphosphorylated, but not hypophosphorylated, RNA polymerase II and CDK p110 PITSLRE kinase via its N-terminal cyclin domains. It can also associate with the SC-35 and 9G8 through its RS repeat region. Recombinant cyclin L2 protein can stimulate in vitro mRNA splicing. Overexpression of human cyclin L2 suppresses the growth of human hepatocellular carcinoma SMMC 7721 cells both in vitro and in vivo, inducing cellular apoptosis. This process involves up-regulation of p53 and Bax and decreased expression of Bcl-2. The data suggest that cyclin L2 represents a new member of the cyclin family, which might regulate the transcription and RNA processing of certain apoptosis-related factors, resulting in tumor cell growth inhibition and apoptosis.
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