Crystallization and preliminary X-ray analysis of a fungal endoglucanase I - PubMed (original) (raw)
Comparative Study
. 1992 Dec 5;228(3):970-2.
doi: 10.1016/0022-2836(92)90879-o.
Affiliations
- PMID: 1469728
- DOI: 10.1016/0022-2836(92)90879-o
Comparative Study
Crystallization and preliminary X-ray analysis of a fungal endoglucanase I
G Davies et al. J Mol Biol. 1992.
Abstract
An endoglucanase I (EG1) from a fungal source (Humicola insolens) has been crystallized in a number of forms suitable for X-ray diffraction analysis. Four crystal forms have been grown from various precipitants using vapour phase diffusion methods in hanging drops. Three of these crystal forms diffract to beyond 2.5 A resolution. Two forms, obtained from ammonium sulphate at pH 5.4, or 8.0, grow as tetragonal bipyramids in space group P4(1)22 or P4(3)22, with approximate cell dimensions a = b = 102 A, c = 282 A. The other crystal forms were grown from polyethylene glycol 8000 at pH 8.0. One grows as monoclinic plates, space group P2(1), with cell dimensions a = 66.9 A, b = 75.2 A, c = 86.9 A and beta = 102.9 degrees and the other as long hexagonal rods in space group P6(1)22 or P6(5)22, with cell dimensions a = b = 119 A, c = 83 A.
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