The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3 - PubMed (original) (raw)
doi: 10.1038/nsmb741. Epub 2004 Mar 7.
Affiliations
- PMID: 15004547
- DOI: 10.1038/nsmb741
The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3
Jan Kadlec et al. Nat Struct Mol Biol. 2004 Apr.
Abstract
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism by which eukaryotic cells detect and degrade transcripts containing premature termination codons. Three 'up-frameshift' proteins, UPF1, UPF2 and UPF3, are essential for this process in organisms ranging from yeast to human. We present a crystal structure at a resolution of 1.95 A of the complex between the interacting domains of human UPF2 and UPF3b, which are, respectively, a MIF4G (middle portion of eIF4G) domain and an RNP domain (ribonucleoprotein-type RNA-binding domain). The protein-protein interface is mediated by highly conserved charged residues in UPF2 and UPF3b and involves the beta-sheet surface of the UPF3b RNP domain, which is generally used by these domains to bind nucleic acids. We show that the UPF3b RNP does not bind RNA, whereas the UPF2 construct and the complex do. Our results advance understanding of the molecular mechanisms underlying the NMD quality control process.
Comment in
- Making structural sense of nonsense-mediated decay.
Lykke-Andersen J. Lykke-Andersen J. Nat Struct Mol Biol. 2004 Apr;11(4):305-6. doi: 10.1038/nsmb0404-305. Nat Struct Mol Biol. 2004. PMID: 15048104 No abstract available.
Similar articles
- Crystal structure of the UPF2-interacting domain of nonsense-mediated mRNA decay factor UPF1.
Kadlec J, Guilligay D, Ravelli RB, Cusack S. Kadlec J, et al. RNA. 2006 Oct;12(10):1817-24. doi: 10.1261/rna.177606. Epub 2006 Aug 24. RNA. 2006. PMID: 16931876 Free PMC article. - Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S. Clerici M, et al. Nucleic Acids Res. 2014 Feb;42(4):2673-86. doi: 10.1093/nar/gkt1197. Epub 2013 Nov 23. Nucleic Acids Res. 2014. PMID: 24271394 Free PMC article. - UPF1 P-body localization.
Brogna S, Ramanathan P, Wen J. Brogna S, et al. Biochem Soc Trans. 2008 Aug;36(Pt 4):698-700. doi: 10.1042/BST0360698. Biochem Soc Trans. 2008. PMID: 18631143 Review. - Role of RNA surveillance proteins Upf1/CpaR, Upf2 and Upf3 in the translational regulation of yeast CPA1 gene.
Messenguy F, Vierendeels F, Piérard A, Delbecq P. Messenguy F, et al. Curr Genet. 2002 Jul;41(4):224-31. doi: 10.1007/s00294-002-0300-4. Epub 2002 Jun 15. Curr Genet. 2002. PMID: 12172963 - Nonsense-mediated mRNA decay: molecular insights and mechanistic variations across species.
Conti E, Izaurralde E. Conti E, et al. Curr Opin Cell Biol. 2005 Jun;17(3):316-25. doi: 10.1016/j.ceb.2005.04.005. Curr Opin Cell Biol. 2005. PMID: 15901503 Review.
Cited by
- The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O. Petit AP, et al. Nucleic Acids Res. 2012 Nov;40(21):11058-72. doi: 10.1093/nar/gks883. Epub 2012 Sep 12. Nucleic Acids Res. 2012. PMID: 22977175 Free PMC article. - An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay.
Jonas S, Weichenrieder O, Izaurralde E. Jonas S, et al. Genes Dev. 2013 Jan 15;27(2):211-25. doi: 10.1101/gad.206672.112. Genes Dev. 2013. PMID: 23348841 Free PMC article. - Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins.
Shirley RL, Richards MR, Culbertson MR. Shirley RL, et al. Biol Proced Online. 2004;6:209-219. doi: 10.1251/bpo91. Epub 2004 Oct 1. Biol Proced Online. 2004. PMID: 15472721 Free PMC article. - Surmounting Cancer Drug Resistance: New Perspective on RNA-Binding Proteins.
Feng Y, Zhu S, Liu T, Zhi G, Shao B, Liu J, Li B, Jiang C, Feng Q, Wu P, Wang D. Feng Y, et al. Pharmaceuticals (Basel). 2023 Aug 7;16(8):1114. doi: 10.3390/ph16081114. Pharmaceuticals (Basel). 2023. PMID: 37631029 Free PMC article. Review. - Nonsense-Mediated mRNA Decay Factor Functions in Human Health and Disease.
Sun L, Mailliot J, Schaffitzel C. Sun L, et al. Biomedicines. 2023 Feb 27;11(3):722. doi: 10.3390/biomedicines11030722. Biomedicines. 2023. PMID: 36979701 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases