Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway - PubMed (original) (raw)
Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway
Bénédicte Py et al. J Immunol. 2004.
Abstract
Siva-1 is a death domain-containing proapoptotic protein identified as an intracellular ligand of CD27 and of the glucocorticoid-induced TNFR family-related gene, which are two members of the TNFR family expressed on lymphoid cells. Although Siva-1 expression is up-regulated in multiple pathological processes, little is known about the signaling pathway underlying the Siva-induced apoptosis. In this study, we investigated the mechanism of the proapoptotic activity of Siva-1 and an alternative splice form lacking the death domain of Siva-1, Siva-2, in T lymphocytes in which Siva proteins, CD27, and glucocorticoid-induced TNFR family-related gene are primarily expressed. Overexpression of Siva proteins triggers a typical apoptotic process manifested by cell shrinkage and surface exposure of phosphatidylserine, and confirmed by ultrastructural features. Siva-induced apoptosis is related to the CD27-mediated apoptotic pathway and results in activation of both initiator and effector caspases. This pathway involves a mitochondrial step evidenced by activation of Bid and cytochrome c release, and is modulated by overexpression of Bcl-2 or Bcl-x(L). The determinants for Siva-induced apoptosis are not contained within the death domain found in the central part of Siva-1, but rather in both the N-terminal and C-terminal regions shared by both Siva proteins. The N-terminal region also participates in the translocation of both Siva proteins into the nuclear compartment. These results indicate that Siva-1 and Siva-2 mediate apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway that likely involves both cytoplasmic and nuclear events.
Similar articles
- Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both bind to CD27 but differentially transduce apoptosis.
Yoon Y, Ao Z, Cheng Y, Schlossman SF, Prasad KV. Yoon Y, et al. Oncogene. 1999 Nov 25;18(50):7174-9. doi: 10.1038/sj.onc.1203144. Oncogene. 1999. PMID: 10597319 - CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein.
Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF. Prasad KV, et al. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6346-51. doi: 10.1073/pnas.94.12.6346. Proc Natl Acad Sci U S A. 1997. PMID: 9177220 Free PMC article. - Signaling of cell death and cell survival following focal cerebral ischemia: life and death struggle in the penumbra.
Ferrer I, Planas AM. Ferrer I, et al. J Neuropathol Exp Neurol. 2003 Apr;62(4):329-39. doi: 10.1093/jnen/62.4.329. J Neuropathol Exp Neurol. 2003. PMID: 12722825 Review. - Activation-induced cell death.
Budd RC. Budd RC. Curr Opin Immunol. 2001 Jun;13(3):356-62. doi: 10.1016/s0952-7915(00)00227-2. Curr Opin Immunol. 2001. PMID: 11406369 Review.
Cited by
- Exposure of Immunogenic Tumor Antigens in Surrendered Immunity and the Significance of Autologous Tumor Cell-Based Vaccination in Precision Medicine.
Ke CH, Chiu YH, Huang KC, Lin CS. Ke CH, et al. Int J Mol Sci. 2022 Dec 21;24(1):147. doi: 10.3390/ijms24010147. Int J Mol Sci. 2022. PMID: 36613591 Free PMC article. Review. - Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the developing mouse cortex.
Sapir T, Kshirsagar A, Gorelik A, Olender T, Porat Z, Scheffer IE, Goldstein DB, Devinsky O, Reiner O. Sapir T, et al. Nat Commun. 2022 Jul 21;13(1):4209. doi: 10.1038/s41467-022-31752-z. Nat Commun. 2022. PMID: 35864088 Free PMC article. - FAIM-L - SIVA-1: Two Modulators of XIAP in Non-Apoptotic Caspase Function.
Coccia E, Solé M, Comella JX. Coccia E, et al. Front Cell Dev Biol. 2022 Jan 10;9:826037. doi: 10.3389/fcell.2021.826037. eCollection 2021. Front Cell Dev Biol. 2022. PMID: 35083225 Free PMC article. Review. - Siva 1 Inhibits Cervical Cancer Progression and Its Clinical Prognosis Significance.
Liu T, Ma Y, Wang Z, Zhang W, Yang X. Liu T, et al. Cancer Manag Res. 2020 Jan 15;12:303-311. doi: 10.2147/CMAR.S232994. eCollection 2020. Cancer Manag Res. 2020. PMID: 32021444 Free PMC article. - SIVA-1 regulates apoptosis and synaptic function by modulating XIAP interaction with the death receptor antagonist FAIM-L.
Coccia E, Planells-Ferrer L, Badillos-Rodríguez R, Pascual M, Segura MF, Fernández-Hernández R, López-Soriano J, Garí E, Soriano E, Barneda-Zahonero B, Moubarak RS, Pérez-García MJ, Comella JX. Coccia E, et al. Cell Death Dis. 2020 Feb 3;11(2):82. doi: 10.1038/s41419-020-2282-x. Cell Death Dis. 2020. PMID: 32015347 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials