CCL21 chemokine regulates chemokine receptor CCR7 bearing malignant melanoma cells - PubMed (original) (raw)
CCL21 chemokine regulates chemokine receptor CCR7 bearing malignant melanoma cells
Hiroya Takeuchi et al. Clin Cancer Res. 2004.
Abstract
Purpose: The chemokine CC-ligand 21/secondary lymphoid tissue chemokine (CCL21/SLC) regulates the homing of naïve T cells and dendritic cells that express CC-chemokine receptor 7 (CCR7) from distant sites to lymphoid tissue such as lymph nodes. We hypothesized that CCL21/SLC regulates the migration of CCR7-bearing melanoma cells from a primary lesion to regional tumor-draining lymph nodes.
Experimental design: Quantitative real-time reverse transcriptase-PCR (qRT) assay and immunohistochemistry (IHC) were used to assess the level of CCR7 expression in melanoma cell lines and in primary and metastatic melanoma tumors. Cell migration assay using melanoma cell lines was performed under the induction of CCL21/SLC. The CCL21/SLC expression level in tumor-draining sentinel lymph nodes (SLNs) was assessed by both qRT assay and IHC.
Results: Melanoma cell lines and tumors demonstrated heterogeneous expression of CCR7 mRNA by qRT assay. There was strong functional correlation between CCR7 mRNA expression and cell migration induced by CCL21/SLC. IHC evidence of CCR7 expression in primary melanomas significantly (P = 0.02) correlated with Breslow thickness. Assessment of SLN from 55 melanoma patients by qRT assay demonstrated that CCL21/SLC mRNA expression level was significantly (P = 0.008) higher in pathologically melanoma-negative SLNs than in melanoma-positive SLNs.
Conclusions: This report demonstrates a potential mechanism for recruitment and homing of CCR7(+) metastatic melanoma cells to tumor-draining lymph nodes, which express CCL21/SLC. The study also suggests that lymph nodes bearing metastasis may suppress CCL21/SLC production.
Similar articles
- Expression of CC chemokine receptor-7 and regional lymph node metastasis of B16 murine melanoma.
Wiley HE, Gonzalez EB, Maki W, Wu MT, Hwang ST. Wiley HE, et al. J Natl Cancer Inst. 2001 Nov 7;93(21):1638-43. doi: 10.1093/jnci/93.21.1638. J Natl Cancer Inst. 2001. PMID: 11698568 - Differential expression of CCL19 by DC-Lamp+ mature dendritic cells in human lymph node versus chronically inflamed skin.
Katou F, Ohtani H, Nakayama T, Nagura H, Yoshie O, Motegi K. Katou F, et al. J Pathol. 2003 Jan;199(1):98-106. doi: 10.1002/path.1255. J Pathol. 2003. PMID: 12474232 - The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7.
Willimann K, Legler DF, Loetscher M, Roos RS, Delgado MB, Clark-Lewis I, Baggiolini M, Moser B. Willimann K, et al. Eur J Immunol. 1998 Jun;28(6):2025-34. doi: 10.1002/(SICI)1521-4141(199806)28:06<2025::AID-IMMU2025>3.0.CO;2-C. Eur J Immunol. 1998. PMID: 9645384 - The multiple personalities of the chemokine receptor CCR7 in dendritic cells.
Sánchez-Sánchez N, Riol-Blanco L, Rodríguez-Fernández JL. Sánchez-Sánchez N, et al. J Immunol. 2006 May 1;176(9):5153-9. doi: 10.4049/jimmunol.176.9.5153. J Immunol. 2006. PMID: 16621978 Review. - A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system.
Comerford I, Harata-Lee Y, Bunting MD, Gregor C, Kara EE, McColl SR. Comerford I, et al. Cytokine Growth Factor Rev. 2013 Jun;24(3):269-83. doi: 10.1016/j.cytogfr.2013.03.001. Epub 2013 Apr 12. Cytokine Growth Factor Rev. 2013. PMID: 23587803 Review.
Cited by
- CCR7 depletion alleviates bony growth imbalance following physeal injury in mice.
Kim W, Sakai Y, Matsuoka M, Hosokawa Y, Fukuda R, Homan K, Onodera T, Iwasaki N. Kim W, et al. Sci Rep. 2024 Oct 22;14(1):24891. doi: 10.1038/s41598-024-75877-1. Sci Rep. 2024. PMID: 39438569 Free PMC article. - High-fat diet, but not duration of lactation, increases mammary gland lymphatic vessel function and subsequent growth of inflammatory breast cancer cells.
Balema W, Morton J, Larson RA, Li L, Velasquez FC, Fowlkes NW, Krishnamurthy S, Debeb BG, Sevick-Muraca E, Woodward WA. Balema W, et al. J Mammary Gland Biol Neoplasia. 2023 Oct 6;28(1):21. doi: 10.1007/s10911-023-09548-8. J Mammary Gland Biol Neoplasia. 2023. PMID: 37801190 Free PMC article. - Immunomodulatory properties of the lymphatic endothelium in the tumor microenvironment.
Viúdez-Pareja C, Kreft E, García-Caballero M. Viúdez-Pareja C, et al. Front Immunol. 2023 Sep 7;14:1235812. doi: 10.3389/fimmu.2023.1235812. eCollection 2023. Front Immunol. 2023. PMID: 37744339 Free PMC article. Review. - Nonclassical monocytes potentiate anti-tumoral CD8+ T cell responses in the lungs.
Padgett LE, Marcovecchio PM, Olingy CE, Araujo DJ, Steel K, Dinh HQ, Alimadadi A, Zhu YP, Meyer MA, Kiosses WB, Thomas GD, Hedrick CC. Padgett LE, et al. Front Immunol. 2023 Jun 22;14:1101497. doi: 10.3389/fimmu.2023.1101497. eCollection 2023. Front Immunol. 2023. PMID: 37426658 Free PMC article. - Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy.
Sah VR, Jespersen H, Karlsson J, Nilsson LM, Bergqvist M, Johansson I, Carneiro A, Helgadottir H, Levin M, Ullenhag G, Ståhlberg A, Olofsson Bagge R, Nilsson JA, Ny L. Sah VR, et al. Cancer Res Commun. 2023 May 18;3(5):884-895. doi: 10.1158/2767-9764.CRC-22-0490. eCollection 2023 May. Cancer Res Commun. 2023. PMID: 37377898 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical