Presence of bacteria and innate immunity of intestinal epithelium in childhood celiac disease - PubMed (original) (raw)
Comparative Study
Presence of bacteria and innate immunity of intestinal epithelium in childhood celiac disease
Göte Forsberg et al. Am J Gastroenterol. 2004 May.
Erratum in
- Am J Gastroenterol. 2004 Jul;99(7):following 1406
Abstract
Objectives: Exposure to gliadin and related prolamins and appropriate HLA-DQ haplotype are necessary but not sufficient for contracting celiac disease (CD). Aberrant innate immune reactions could be contributing risk factors. Therefore, jejunal biopsies were screened for bacteria and the innate immune status of the epithelium investigated.
Methods: Children with untreated, treated, challenged CD, and controls were analyzed. Bacteria were identified by scanning electron microscopy. Glycocalyx composition and mucin and antimicrobial peptide production were studied by quantitative RT-PCR, antibody and lectin immunohistochemistry.
Results: Rod-shaped bacteria were frequently associated with the mucosa of CD patients, with both active and inactive disease, but not with controls. The lectin Ulex europaeus agglutinin I (UEAI) stained goblet cells in the mucosa of all CD patients but not of controls. The lectin peanut agglutinin (PNA) stained glycocalyx of controls but not of CD patients. mRNA levels of mucin-2 (MUC2), alpha-defensins HD-5 and HD-6, and lysozyme were significantly increased in active CD and returned to normal in treated CD. Their expression levels correlated to the interferon-gamma mRNA levels in intraepithelial lymphocytes. MUC2, HD-5, and lysozyme proteins were seen in absorptive epithelial cells. beta-defensins hBD-1 and hBD-2, carcinoembryonic antigen (CEA), CEA cell adhesion molecule-1a (CEACAM1a), and MUC3 were not affected.
Conclusions: Unique carbohydrate structures of the glycocalyx/mucous layer are likely discriminating features of CD patients. These glycosylation differences could facilitate bacterial adhesion. Ectopic production of MUC2, HD-5, and lysozyme in active CD is compatible with goblet and Paneth cell metaplasia induced by high interferon-gamma production by intraepithelial lymphocytes.
Comment in
- A role for bacteria in celiac disease?
Sollid LM, Gray GM. Sollid LM, et al. Am J Gastroenterol. 2004 May;99(5):905-6. doi: 10.1111/j.1572-0241.2004.04158.x. Am J Gastroenterol. 2004. PMID: 15128358
Similar articles
- Proximal small intestinal microbiota and identification of rod-shaped bacteria associated with childhood celiac disease.
Ou G, Hedberg M, Hörstedt P, Baranov V, Forsberg G, Drobni M, Sandström O, Wai SN, Johansson I, Hammarström ML, Hernell O, Hammarström S. Ou G, et al. Am J Gastroenterol. 2009 Dec;104(12):3058-67. doi: 10.1038/ajg.2009.524. Epub 2009 Sep 15. Am J Gastroenterol. 2009. PMID: 19755974 - A role for bacteria in celiac disease?
Sollid LM, Gray GM. Sollid LM, et al. Am J Gastroenterol. 2004 May;99(5):905-6. doi: 10.1111/j.1572-0241.2004.04158.x. Am J Gastroenterol. 2004. PMID: 15128358 - Contribution of intestinal epithelial cells to innate immunity of the human gut--studies on polarized monolayers of colon carcinoma cells.
Ou G, Baranov V, Lundmark E, Hammarström S, Hammarström ML. Ou G, et al. Scand J Immunol. 2009 Feb;69(2):150-61. doi: 10.1111/j.1365-3083.2008.02208.x. Scand J Immunol. 2009. PMID: 19170965 - Adaptive and innate immune responses in celiac disease.
Gianfrani C, Auricchio S, Troncone R. Gianfrani C, et al. Immunol Lett. 2005 Jul 15;99(2):141-5. doi: 10.1016/j.imlet.2005.02.017. Epub 2005 Apr 7. Immunol Lett. 2005. PMID: 15876458 Review. - Gliadin as a stimulator of innate responses in celiac disease.
Londei M, Ciacci C, Ricciardelli I, Vacca L, Quaratino S, Maiuri L. Londei M, et al. Mol Immunol. 2005 May;42(8):913-8. doi: 10.1016/j.molimm.2004.12.005. Epub 2005 Jan 11. Mol Immunol. 2005. PMID: 15829281 Review.
Cited by
- The intermicrovillar adhesion complex in gut barrier function and inflammation.
Mödl B, Schmidt K, Moser D, Eferl R. Mödl B, et al. Explor Dig Dis. 2022 Oct 11;1:72-79. doi: 10.37349/edd.2022.00006. Explor Dig Dis. 2022. PMID: 39092422 Free PMC article. - The Emerging Role of Innate Lymphoid Cells (ILCs) and Alarmins in Celiac Disease: An Update on Pathophysiological Insights, Potential Use as Disease Biomarkers, and Therapeutic Implications.
Rizzi A, Di Gioacchino M, Gammeri L, Inchingolo R, Chini R, Santilli F, Nucera E, Gangemi S. Rizzi A, et al. Cells. 2023 Jul 21;12(14):1910. doi: 10.3390/cells12141910. Cells. 2023. PMID: 37508573 Free PMC article. Review. - Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the faecal metabolome in children with coeliac disease autoimmunity: a randomised, double-blinded placebo-controlled clinical trial.
Jenickova E, Andrén Aronsson C, Mascellani Bergo A, Cinek O, Havlik J, Agardh D. Jenickova E, et al. Front Nutr. 2023 Jul 6;10:1183963. doi: 10.3389/fnut.2023.1183963. eCollection 2023. Front Nutr. 2023. PMID: 37485388 Free PMC article. - The Activity of Antimicrobial Peptides in Pediatric Celiac Disease.
Kamilova AT, Azizova GK, Umarnazarova ZE, Abdullaeva DA, Geller SI. Kamilova AT, et al. Front Pediatr. 2022 Jun 6;10:873793. doi: 10.3389/fped.2022.873793. eCollection 2022. Front Pediatr. 2022. PMID: 35733815 Free PMC article. - Molecular and Structural Parallels between Gluten Pathogenic Peptides and Bacterial-Derived Proteins by Bioinformatics Analysis.
Vazquez DS, Schilbert HM, Dodero VI. Vazquez DS, et al. Int J Mol Sci. 2021 Aug 27;22(17):9278. doi: 10.3390/ijms22179278. Int J Mol Sci. 2021. PMID: 34502187 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous