Immunoglobulin coating of faecal bacteria in inflammatory bowel disease - PubMed (original) (raw)
Immunoglobulin coating of faecal bacteria in inflammatory bowel disease
Laurens A van der Waaij et al. Eur J Gastroenterol Hepatol. 2004 Jul.
Abstract
Objective: An inappropriate mucosal immune response to the commensal bacterial flora may play a role in the pathogenesis of inflammatory bowel disease (IBD). In this study we determined the percentage of immunoglobulin-coated bacteria in the stools of patients and controls.
Methods: Faecal samples were obtained from 18 patients with IBD (one sample during exacerbation and one shortly after remission was achieved), 15 healthy volunteers, eight infectious colitis patients, and 13 IBD patients in long-term remission. Bacterial immunoglobulin coating was determined by flow-cytometry analysis. Faecal alpha-1-antitrypsin concentrations were determined by radial immune diffusion.
Results: IBD patients had 69 +/- 19% immunoglobulin A (IgA)-, 56 +/- 32% immunoglobulin G (IgG)- and 56 +/- 29% immunoglobulin M (IgM)-coated bacteria in their faeces. Healthy controls had less immunoglobulin coating, respectively 36 +/- 12%, 11 +/- 4% and 11 +/- 7%. Infectious colitis patients had 57 +/- 14% IgA, 31 +/- 13% IgG, and 42 +/-16% IgM; however, they had higher faecal alpha-1-antitrypsin concentrations than IBD patients. Shortly after remission, IBD patients had 65 +/- 20% IgA, 32 +/- 18% IgG and 40 +/- 21% IgM. Long-term-remission IBD patients had normal IgG and IgM but increased IgA (50 +/- 16%) coating.
Conclusions: Compared with healthy controls, patients with IBD had an increased percentage of immunoglobulin-coated faecal anaerobic bacteria, both in active disease and shortly after remission. These results support the concept that there may be a breakdown of mucosal tolerance to the commensal gut flora in IBD.
Similar articles
- Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease.
Lin R, Chen H, Shu W, Sun M, Fang L, Shi Y, Pang Z, Wu W, Liu Z. Lin R, et al. J Transl Med. 2018 Dec 17;16(1):359. doi: 10.1186/s12967-018-1723-0. J Transl Med. 2018. PMID: 30558634 Free PMC article. - Mucosal antibodies in inflammatory bowel disease are directed against intestinal bacteria.
Macpherson A, Khoo UY, Forgacs I, Philpott-Howard J, Bjarnason I. Macpherson A, et al. Gut. 1996 Mar;38(3):365-75. doi: 10.1136/gut.38.3.365. Gut. 1996. PMID: 8675088 Free PMC article. - In vivo IgA coating of anaerobic bacteria in human faeces.
van der Waaij LA, Limburg PC, Mesander G, van der Waaij D. van der Waaij LA, et al. Gut. 1996 Mar;38(3):348-54. doi: 10.1136/gut.38.3.348. Gut. 1996. PMID: 8675085 Free PMC article. - Bridging the gap between host immune response and intestinal dysbiosis in inflammatory bowel disease: does immunoglobulin A mark the spot?
Shapiro JM, Cho JH, Sands BE, LeLeiko NS. Shapiro JM, et al. Clin Gastroenterol Hepatol. 2015 May;13(5):842-6. doi: 10.1016/j.cgh.2015.02.028. Epub 2015 Feb 25. Clin Gastroenterol Hepatol. 2015. PMID: 25725444 Review. - Rethinking mucosal antibody responses: IgM, IgG and IgD join IgA.
Chen K, Magri G, Grasset EK, Cerutti A. Chen K, et al. Nat Rev Immunol. 2020 Jul;20(7):427-441. doi: 10.1038/s41577-019-0261-1. Epub 2020 Feb 3. Nat Rev Immunol. 2020. PMID: 32015473 Free PMC article. Review.
Cited by
- Enteral feeding reduces metabolic activity of the intestinal microbiome in Crohn's disease: an observational study.
Walton C, Montoya MP, Fowler DP, Turner C, Jia W, Whitehead RN, Griffiths L, Waring RH, Ramsden DB, Cole JA, Cauchi M, Bessant C, Naylor SJ, Hunter JO. Walton C, et al. Eur J Clin Nutr. 2016 Sep;70(9):1052-6. doi: 10.1038/ejcn.2016.74. Epub 2016 May 11. Eur J Clin Nutr. 2016. PMID: 27167669 Clinical Trial. - Elemental diet and the nutritional treatment of Crohn's disease.
Hunter J. Hunter J. Gastroenterol Hepatol Bed Bench. 2015 Winter;8(1):4-5. Gastroenterol Hepatol Bed Bench. 2015. PMID: 25584170 Free PMC article. No abstract available. - Clinical significance of soluble immunoglobulins A and G and their coated bacteria in feces of patients with inflammatory bowel disease.
Lin R, Chen H, Shu W, Sun M, Fang L, Shi Y, Pang Z, Wu W, Liu Z. Lin R, et al. J Transl Med. 2018 Dec 17;16(1):359. doi: 10.1186/s12967-018-1723-0. J Transl Med. 2018. PMID: 30558634 Free PMC article. - IgA and FcαRI: Pathological Roles and Therapeutic Opportunities.
Breedveld A, van Egmond M. Breedveld A, et al. Front Immunol. 2019 Mar 22;10:553. doi: 10.3389/fimmu.2019.00553. eCollection 2019. Front Immunol. 2019. PMID: 30984170 Free PMC article. Review. - Methods in microbiome research: Past, present, and future.
Gotschlich EC, Colbert RA, Gill T. Gotschlich EC, et al. Best Pract Res Clin Rheumatol. 2019 Dec;33(6):101498. doi: 10.1016/j.berh.2020.101498. Epub 2020 Apr 24. Best Pract Res Clin Rheumatol. 2019. PMID: 32340923 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous