Role of phosphorylation in p34cdc2 activation: identification of an activating kinase - PubMed (original) (raw)
Role of phosphorylation in p34cdc2 activation: identification of an activating kinase
M J Solomon et al. Mol Biol Cell. 1992 Jan.
Free PMC article
Abstract
Phosphorylation of p34cdc2 can both positively and negatively regulate its kinase activity. We have mapped two phosphorylation sites in Xenopus p34cdc2 to Thr-14 and Tyr-15 within the putative ATP-binding region of p34cdc2. Mutation of these sites to Ala-14 and Phe-15 has no effect on the final histone H1 kinase activity of the cyclin/p34cdc2 complex. Phosphopeptide analysis shows that there is at least one more site of phosphorylation on p34cdc2. When Thr-161 is changed to Ala, two phosphopeptide spots disappear and it is no longer possible to activate the H1 kinase activity of p34cdc2. We suggest that Thr-161 is a third site of phosphorylation, which is required for kinase activity. All three phosphorylations are induced by cyclin. None of the phosphorylations appears to be required for binding to cyclin, as indicated by the ability of the triple mutant, Ala-14, Phe-15, Ala-161, to bind cyclin. The activating phosphorylation that requires Thr- or Ser-161 occurs even in a catalytically inactive K33R mutant of p34cdc2 and hence does not appear to be the result of intramolecular autophosphorylation. We have detected an activity in Xenopus extracts required for activation of p34cdc2 and present evidence that this is a p34cdc2 activating kinase which, in a cyclin-dependent manner, probably directly phosphorylates Thr-161.
Similar articles
- Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites.
Pickham KM, Meyer AN, Li J, Donoghue DJ. Pickham KM, et al. Mol Cell Biol. 1992 Jul;12(7):3192-203. doi: 10.1128/mcb.12.7.3192-3203.1992. Mol Cell Biol. 1992. PMID: 1377775 Free PMC article. - Mechanisms of p34cdc2 regulation.
Atherton-Fessler S, Parker LL, Geahlen RL, Piwnica-Worms H. Atherton-Fessler S, et al. Mol Cell Biol. 1993 Mar;13(3):1675-85. doi: 10.1128/mcb.13.3.1675-1685.1993. Mol Cell Biol. 1993. PMID: 8441405 Free PMC article. - Tyrosine phosphorylation of p34cdc2 is regulated by protein phosphatase 2A in growing immature Xenopus oocytes.
Rime H, Jessus C, Ozon R. Rime H, et al. Exp Cell Res. 1995 Jul;219(1):29-38. doi: 10.1006/excr.1995.1201. Exp Cell Res. 1995. PMID: 7543054 - Regulation of p34cdc2 protein kinase activity by phosphorylation and cyclin binding.
Nigg EA, Gallant P, Krek W. Nigg EA, et al. Ciba Found Symp. 1992;170:72-84; discussion 84-96. doi: 10.1002/9780470514320.ch6. Ciba Found Symp. 1992. PMID: 1483352 Review. - [From ovocyte to biochemistry of the cell cycle].
Ozon R. Ozon R. Verh K Acad Geneeskd Belg. 1991;53(4):365-85. Verh K Acad Geneeskd Belg. 1991. PMID: 1659057 Review. French.
Cited by
- Profiling Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay.
Gassaway BM, Huttlin EL, Huntsman EM, Yaron-Barir TM, Johnson JL, Kurmi K, Cantley LC, Paulo JA, Ringel AE, Gygi SP, Haigis MC. Gassaway BM, et al. Mol Cell Proteomics. 2024 Jul;23(7):100801. doi: 10.1016/j.mcpro.2024.100801. Epub 2024 Jun 15. Mol Cell Proteomics. 2024. PMID: 38880243 Free PMC article. - Hyperactivation of p53 contributes to mitotic catastrophe in podocytes through regulation of the Wee1/CDK1/cyclin B1 axis.
Feng J, Xie L, Lu W, Yu X, Dong H, Ma Y, Kong R. Feng J, et al. Ren Fail. 2024 Dec;46(2):2365408. doi: 10.1080/0886022X.2024.2365408. Epub 2024 Jun 14. Ren Fail. 2024. PMID: 38874119 Free PMC article. - Exploring the conformational landscapes of protein kinases: perspectives from FRET and DEER.
Baker ZD, Rasmussen DM, Levinson NM. Baker ZD, et al. Biochem Soc Trans. 2024 Jun 26;52(3):1071-1083. doi: 10.1042/BST20230558. Biochem Soc Trans. 2024. PMID: 38778760 Free PMC article. Review. - Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro.
Shintomi K, Masahara-Negishi Y, Shima M, Tane S, Hirano T. Shintomi K, et al. PLoS One. 2024 Mar 25;19(3):e0299003. doi: 10.1371/journal.pone.0299003. eCollection 2024. PLoS One. 2024. PMID: 38527022 Free PMC article. - Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence.
Valencia C, Pérez-García F, Aguila L, Felmer R, Arias ME. Valencia C, et al. Int J Mol Sci. 2023 Oct 31;24(21):15794. doi: 10.3390/ijms242115794. Int J Mol Sci. 2023. PMID: 37958778 Free PMC article.
References
- Mol Cell Biol. 1991 Aug;11(8):3860-7 - PubMed
- EMBO J. 1991 Jun;10(6):1545-54 - PubMed
- Cell. 1991 Mar 8;64(5):903-14 - PubMed
- EMBO J. 1991 Feb;10(2):305-16 - PubMed
- Science. 1991 Jul 26;253(5018):414-20 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous