The retinoblastoma protein physically associates with the human cdc2 kinase - PubMed (original) (raw)

The retinoblastoma protein physically associates with the human cdc2 kinase

Q J Hu et al. Mol Cell Biol. 1992 Mar.

Abstract

The protein product (pRB) of the retinoblastoma susceptibility gene functions as a negative regulator of cell proliferation, and its activity appears to be modulated by phosphorylation. Using a new panel of anti-human pRB monoclonal antibodies, we have investigated the biochemical properties of this protein. These antibodies have allowed us to detect a pRB-associated kinase that has been identified as the cell cycle-regulating kinase p34cdc2 or a closely related enzyme. Since this associated kinase phosphorylates pRB at most of the sites used in vivo, these results suggest that this kinase is one of the major regulators of pRB. The associated kinase activity follows the pattern of phosphorylation seen for pRB in vivo. The associated kinase activity is not seen in the G1 phase but appears in the S phase, and the levels continue to increase throughout the remainder of the cell cycle.

PubMed Disclaimer

References

    1. Cell. 1989 Sep 22;58(6):1097-105 - PubMed
    1. Cell. 1989 Sep 22;58(6):1193-8 - PubMed
    1. Oncogene. 1989 Jul;4(7):839-43 - PubMed
    1. EMBO J. 1987 Oct;6(10):2987-95 - PubMed
    1. Science. 1988 Jul 15;241(4863):353-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources