Multiple effects of hyperbaric oxygen on the expression of HIF-1 alpha and apoptotic genes in a global ischemia-hypotension rat model - PubMed (original) (raw)

Comparative Study

Multiple effects of hyperbaric oxygen on the expression of HIF-1 alpha and apoptotic genes in a global ischemia-hypotension rat model

Yun Li et al. Exp Neurol. 2005 Jan.

Abstract

Hypoxia-inducible factor-1alpha (HIF-1alpha) is a transcription factor specifically activated by hypoxia. Activation of proapoptotic caspase-9 and caspase-3 pathways, by binding with tumor suppressor p53, HIF-1alpha could lead to harmful actions such as apoptosis. We examined whether increasing oxygen levels by hyperbaric oxygen (HBO) offers neuroprotection, at least partially by suppression of HIF-1alpha and apoptotic genes. Male SD rats (n = 78) were randomly divided into 13 groups: 1 sham group, 6 groups of global ischemia-hypotension (GI), and 6 groups of HBO treatment after global ischemia-hypotension (GI + HBO). HBO (3 ATA for 2 h) was applied at 1 h after global ischemia-hypotension. Rats were sacrificed at 6, 12, 24, 48, and 96 h and 7 days. Global ischemia-hypotension (10 min ischemia, 30-35 mm Hg) produced a marked increase of HIF-1alpha expressions in the hippocampus and cortex at 6 h and peaked at 48-96 h. The expressions of p53, caspase-9, and caspase-3 were all increased in a similar time course. These molecular changes were accompanied by massive cell loss in the hippocampal regions and to a lesser degree in the cortex, with features of apoptosis. HBO treatment reduced expressions of HIF-1alpha, p53, caspase-9, and caspase-3 and decreased cell death. The protein levels of proapoptotic caspase-8 and antiapoptotic bcl-2 were increased after global ischemia-hypotension and HBO potentiated the expression of caspase-8 and decreased expression of bcl-2. These results indicate that HBO has multiple actions on apoptotic genes even though the overall effect of HBO was decreased HIF-1alpha expression and reduced apoptosis after global ischemia-hypotension.

PubMed Disclaimer

Cited by

Oxidative stress is fundamental to hyperbaric oxygen therapy.
Thom SR. Thom SR. J Appl Physiol (1985). 2009 Mar;106(3):988-95. doi: 10.1152/japplphysiol.91004.2008. Epub 2008 Oct 9. J Appl Physiol (1985). 2009. PMID: 18845776 Free PMC article. Review.
Hyperbaric oxygen and hyperbaric air treatment result in comparable neuronal death reduction and improved behavioral outcome after transient forebrain ischemia in the gerbil.
Malek M, Duszczyk M, Zyszkowski M, Ziembowicz A, Salinska E. Malek M, et al. Exp Brain Res. 2013 Jan;224(1):1-14. doi: 10.1007/s00221-012-3283-5. Epub 2012 Oct 2. Exp Brain Res. 2013. PMID: 23283415 Free PMC article.
Hyperbaric oxygen prevents early death caused by experimental cerebral malaria.
Blanco YC, Farias AS, Goelnitz U, Lopes SC, Arrais-Silva WW, Carvalho BO, Amino R, Wunderlich G, Santos LM, Giorgio S, Costa FT. Blanco YC, et al. PLoS One. 2008 Sep 4;3(9):e3126. doi: 10.1371/journal.pone.0003126. PLoS One. 2008. PMID: 18769544 Free PMC article.
Hyperbaric oxygen therapy in acute ischemic stroke: a review.
Ding Z, Tong WC, Lu XX, Peng HP. Ding Z, et al. Interv Neurol. 2014 Aug;2(4):201-11. doi: 10.1159/000362677. Interv Neurol. 2014. PMID: 25337089 Free PMC article. Review.
Is HOT a cool treatment for type 1 diabetes?
Rajagopalan G, Kudva YC, David CS. Rajagopalan G, et al. Diabetes. 2012 Jul;61(7):1664-6. doi: 10.2337/db12-0527. Diabetes. 2012. PMID: 22723274 Free PMC article. No abstract available.

Multiple effects of hyperbaric oxygen on the expression of HIF-1 alpha and apoptotic genes in a global ischemia-hypotension rat model - PubMed (original) (raw)