The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1 - PubMed (original) (raw)
. 2005 Mar 18;280(11):9879-86.
doi: 10.1074/jbc.M414453200. Epub 2005 Jan 4.
Affiliations
- PMID: 15632126
- DOI: 10.1074/jbc.M414453200
Free article
The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1
Michele Giannattasio et al. J Biol Chem. 2005.
Free article
Abstract
The cellular response to DNA lesions entails the recruitment of several checkpoint and repair factors to damaged DNA, and chromatin modifications may play a role in this process. Here we show that in Saccharomyces cerevisiae epigenetic modification of histones is required for checkpoint activity in response to a variety of genotoxic stresses. We demonstrate that ubiquitination of histone H2B on lysine 123 by the Rad6-Bre1 complex, is necessary for activation of Rad53 kinase and cell cycle arrest. We found a similar requirement for Dot1-dependent methylation of histone H3. Loss of H3-Lys(79) methylation does not affect Mec1 activation, whereas it renders cells checkpoint-defective by preventing phosphorylation of Rad9. Such results suggest that histone modifications may have a role in checkpoint function by modulating the interactions of Rad9 with chromatin and active Mec1 kinase.
Similar articles
- Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9.
Wysocki R, Javaheri A, Allard S, Sha F, Côté J, Kron SJ. Wysocki R, et al. Mol Cell Biol. 2005 Oct;25(19):8430-43. doi: 10.1128/MCB.25.19.8430-8443.2005. Mol Cell Biol. 2005. PMID: 16166626 Free PMC article. - Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation.
Toh GW, O'Shaughnessy AM, Jimeno S, Dobbie IM, Grenon M, Maffini S, O'Rorke A, Lowndes NF. Toh GW, et al. DNA Repair (Amst). 2006 Jun 10;5(6):693-703. doi: 10.1016/j.dnarep.2006.03.005. Epub 2006 May 2. DNA Repair (Amst). 2006. PMID: 16650810 - The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS.
Wood A, Schneider J, Dover J, Johnston M, Shilatifard A. Wood A, et al. Mol Cell. 2005 Nov 23;20(4):589-99. doi: 10.1016/j.molcel.2005.09.010. Mol Cell. 2005. PMID: 16307922 - The role of RAD6 in recombinational repair, checkpoints and meiosis via histone modification.
Game JC, Chernikova SB. Game JC, et al. DNA Repair (Amst). 2009 Apr 5;8(4):470-82. doi: 10.1016/j.dnarep.2009.01.007. Epub 2009 Feb 18. DNA Repair (Amst). 2009. PMID: 19230796 Review. - The Bre1/Rad6 machinery: writing the central histone ubiquitin mark on H2B and beyond.
Deng ZH, Ai HS, Lu CP, Li JB. Deng ZH, et al. Chromosome Res. 2020 Dec;28(3-4):247-258. doi: 10.1007/s10577-020-09640-3. Epub 2020 Sep 7. Chromosome Res. 2020. PMID: 32895784 Review.
Cited by
- DOT1L-mediated RAP80 methylation promotes BRCA1 recruitment to elicit DNA repair.
Tang H, Lu YF, Zeng R, Liu C, Shu Y, Wu Y, Su J, Di L, Qian J, Zhang J, Tian Y, Lu X, Pei XH, Zhu Q, Zhu WG. Tang H, et al. Proc Natl Acad Sci U S A. 2024 Aug 27;121(35):e2320804121. doi: 10.1073/pnas.2320804121. Epub 2024 Aug 22. Proc Natl Acad Sci U S A. 2024. PMID: 39172790 - A novel role of DOT1L in kidney diseases.
Wei W, Zhao Y, Chai Y, Shou S, Jin H. Wei W, et al. Mol Biol Rep. 2023 Jun;50(6):5415-5423. doi: 10.1007/s11033-023-08415-3. Epub 2023 Apr 22. Mol Biol Rep. 2023. PMID: 37085741 Review. - Histone modification in Saccharomyces cerevisiae: A review of the current status.
Chou KY, Lee JY, Kim KB, Kim E, Lee HS, Ryu HY. Chou KY, et al. Comput Struct Biotechnol J. 2023 Feb 24;21:1843-1850. doi: 10.1016/j.csbj.2023.02.037. eCollection 2023. Comput Struct Biotechnol J. 2023. PMID: 36915383 Free PMC article. Review. - The Chromatin Landscape around DNA Double-Strand Breaks in Yeast and Its Influence on DNA Repair Pathway Choice.
Frigerio C, Di Nisio E, Galli M, Colombo CV, Negri R, Clerici M. Frigerio C, et al. Int J Mol Sci. 2023 Feb 7;24(4):3248. doi: 10.3390/ijms24043248. Int J Mol Sci. 2023. PMID: 36834658 Free PMC article. Review. - The DNA damage checkpoint: A tale from budding yeast.
Pizzul P, Casari E, Gnugnoli M, Rinaldi C, Corallo F, Longhese MP. Pizzul P, et al. Front Genet. 2022 Sep 15;13:995163. doi: 10.3389/fgene.2022.995163. eCollection 2022. Front Genet. 2022. PMID: 36186482 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials