Hemifusion in SNARE-mediated membrane fusion - PubMed (original) (raw)
doi: 10.1038/nsmb921. Epub 2005 Apr 10.
Affiliations
- PMID: 15821745
- DOI: 10.1038/nsmb921
Hemifusion in SNARE-mediated membrane fusion
Yibin Xu et al. Nat Struct Mol Biol. 2005 May.
Abstract
SNAREs are essential for intracellular membrane fusion. Using EPR, we determined the structure of the transmembrane domain (TMD) of the vesicle (v)-SNARE Snc2p involved in trafficking in yeast. Structural features of the TMD were used to design a v-SNARE mutant in which about half of the TMD was deleted. Liposomes containing this mutant induced outer leaflet mixing but not inner leaflet mixing when incubated with liposomes containing target membrane (t)-SNAREs. Hemifusion was also detected with wild-type SNAREs when low protein concentrations were reconstituted. Thus, these results show that SNARE-mediated fusion can transit through a hemifusion intermediate.
Comment in
- Searching for the silver lining.
White JM, Castle JD. White JM, et al. Nat Struct Mol Biol. 2005 May;12(5):382-4. doi: 10.1038/nsmb0505-382. Nat Struct Mol Biol. 2005. PMID: 15870723 No abstract available.
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