Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase - PubMed (original) (raw)
doi: 10.1038/ncb1262. Epub 2005 May 15.
Affiliations
- PMID: 15895076
- DOI: 10.1038/ncb1262
Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
Ellen J Ezratty et al. Nat Cell Biol. 2005 Jun.
Abstract
Imaging studies implicate microtubule targeting of focal adhesions in focal adhesion disassembly, although the molecular mechanism is unknown. Here, we develop a model system of focal adhesion disassembly based on the finding that microtubule regrowth after nocodazole washout induces disassembly of focal adhesions, and that this disassembly occurs independently of Rho and Rac, but depends on focal adhesion kinase (FAK) and dynamin. During disassembly, dynamin interacts with FAK and colocalizes with focal adhesions. Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation.
Comment in
- Foot in mouth: do focal adhesions disassemble by endocytosis?
Burridge K. Burridge K. Nat Cell Biol. 2005 Jun;7(6):545-7. doi: 10.1038/ncb0505-545. Nat Cell Biol. 2005. PMID: 15928699 No abstract available.
Similar articles
- Regulation of focal adhesion dynamics and disassembly by phosphorylation of FAK at tyrosine 397.
Hamadi A, Bouali M, Dontenwill M, Stoeckel H, Takeda K, Rondé P. Hamadi A, et al. J Cell Sci. 2005 Oct 1;118(Pt 19):4415-25. doi: 10.1242/jcs.02565. Epub 2005 Sep 13. J Cell Sci. 2005. PMID: 16159962 - Localized stabilization of microtubules by integrin- and FAK-facilitated Rho signaling.
Palazzo AF, Eng CH, Schlaepfer DD, Marcantonio EE, Gundersen GG. Palazzo AF, et al. Science. 2004 Feb 6;303(5659):836-9. doi: 10.1126/science.1091325. Science. 2004. PMID: 14764879 - Asymmetric focal adhesion disassembly in motile cells.
Broussard JA, Webb DJ, Kaverina I. Broussard JA, et al. Curr Opin Cell Biol. 2008 Feb;20(1):85-90. doi: 10.1016/j.ceb.2007.10.009. Curr Opin Cell Biol. 2008. PMID: 18083360 Review. - Fak and focal adhesions.
Martin GM. Martin GM. Jpn J Cancer Res. 1996 Mar;87(3):inside front cover. Jpn J Cancer Res. 1996. PMID: 8613421 Review. No abstract available.
Cited by
- Myosin 1e deficiency affects migration of 4T1 breast cancer cells.
Garone ME, Chase SE, Zhang C, Krendel M. Garone ME, et al. Cytoskeleton (Hoboken). 2024 Dec;81(12):723-736. doi: 10.1002/cm.21819. Epub 2023 Dec 23. Cytoskeleton (Hoboken). 2024. PMID: 38140937 - The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.
Whitelaw JA, Swaminathan K, Kage F, Machesky LM. Whitelaw JA, et al. Cells. 2020 Jul 7;9(7):1635. doi: 10.3390/cells9071635. Cells. 2020. PMID: 32646006 Free PMC article. - Alterations in ovarian cancer cell adhesion drive taxol resistance by increasing microtubule dynamics in a FAK-dependent manner.
McGrail DJ, Khambhati NN, Qi MX, Patel KS, Ravikumar N, Brandenburg CP, Dawson MR. McGrail DJ, et al. Sci Rep. 2015 Apr 17;5:9529. doi: 10.1038/srep09529. Sci Rep. 2015. PMID: 25886093 Free PMC article. - Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53.
de Queiroz RM, Efe G, Guzman A, Hashimoto N, Kawashima Y, Tanaka T, Rustgi AK, Prives C. de Queiroz RM, et al. Nat Commun. 2024 Aug 20;15(1):7132. doi: 10.1038/s41467-024-51488-2. Nat Commun. 2024. PMID: 39164253 Free PMC article. - Scaffold mediated regulation of MAPK signaling and cytoskeletal dynamics: a perspective.
Pullikuth AK, Catling AD. Pullikuth AK, et al. Cell Signal. 2007 Aug;19(8):1621-32. doi: 10.1016/j.cellsig.2007.04.012. Epub 2007 May 5. Cell Signal. 2007. PMID: 17553668 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous