Cloning, expression and characterization of human glutathione S-transferase Omega 2 - PubMed (original) (raw)
Affiliations
- PMID: 15942673
Cloning, expression and characterization of human glutathione S-transferase Omega 2
Liu Wang et al. Int J Mol Med. 2005 Jul.
Abstract
The class of Omega glutathione transferases is newly identified with novel structural and functional characteristics. Human GSTO 1-1 (glutathione S-transferase Omega 1) is the first member of the GST Omega class. It was found to play a role in apoptosis and be in association with age-at-onset of AD and PD. In order to improve the understanding of the properties of other Omega class members, we screened a human fetal brain cDNA library and obtained the human GSTO2 (glutathione S-transferase Omega 2) cDNA. The full-length cDNA of human GSTO2 is 1179 bp long and encodes a protein of 243 amino acid residues. Expression pattern analysis revealed that GSTO2 was ubiquitously expressed at a low level, with a higher expression in pancreas and prostate. Enzyme assays showed that GSTO2 protein had activities similar to Omega class GSTs. It has detectable glutathione-dependent thiol transferase activity and glutathione-dependent dehydroascorbate reductase activity. But different from GSTO1-1, GSTO2 exhibits a high catalytic activity with CDNB. Subcellular localization analysis of GSTO2-EGFP fusion protein revealed that GSTO2 distributed to cytoplasm of COS-7 cells and both cytoplasm and nucleus of L-02, QGY-7703 and SMMC-7721 cells. Overexpression of GSTO2 induced apoptosis of L-02 cells detected by Annexin V-PE staining. The results suggest that GSTO2 may play an important role in cellular signaling.
Similar articles
- Novel omega glutathione S-transferases in disk abalone: Characterization and protective roles against environmental stress.
Wan Q, Whang I, Lee JS, Lee J. Wan Q, et al. Comp Biochem Physiol C Toxicol Pharmacol. 2009 Nov;150(4):558-68. doi: 10.1016/j.cbpc.2009.08.003. Epub 2009 Aug 15. Comp Biochem Physiol C Toxicol Pharmacol. 2009. PMID: 19689930 - Characterization of the omega class of glutathione transferases.
Whitbread AK, Masoumi A, Tetlow N, Schmuck E, Coggan M, Board PG. Whitbread AK, et al. Methods Enzymol. 2005;401:78-99. doi: 10.1016/S0076-6879(05)01005-0. Methods Enzymol. 2005. PMID: 16399380 Review. - Molecular characterization of a glutathione transferase from Pinus tabulaeformis (Pinaceae).
Zeng QY, Lu H, Wang XR. Zeng QY, et al. Biochimie. 2005 May;87(5):445-55. doi: 10.1016/j.biochi.2005.01.002. Biochimie. 2005. PMID: 15820751 - The omega-class glutathione transferases: structure, function, and genetics.
Board PG. Board PG. Drug Metab Rev. 2011 May;43(2):226-35. doi: 10.3109/03602532.2011.561353. Drug Metab Rev. 2011. PMID: 21495794 Review.
Cited by
- Female Germ Cell Development in Chickens and Humans: The Chicken Oocyte Enriched Genes Convergent and Divergent with the Human Oocyte.
Rengaraj D, Han JY. Rengaraj D, et al. Int J Mol Sci. 2022 Sep 27;23(19):11412. doi: 10.3390/ijms231911412. Int J Mol Sci. 2022. PMID: 36232712 Free PMC article. Review. - Loss of GSTO2 contributes to cell growth and mitochondria function via the p38 signaling in lung squamous cell carcinoma.
Sumiya R, Terayama M, Hagiwara T, Nakata K, Sekihara K, Nagasaka S, Miyazaki H, Igari T, Yamada K, Kawamura YI. Sumiya R, et al. Cancer Sci. 2022 Jan;113(1):195-204. doi: 10.1111/cas.15189. Epub 2021 Nov 21. Cancer Sci. 2022. PMID: 34726807 Free PMC article. - GSTT1 null and rs156697 Polymorphism in GSTO2 Influence the Risk and Therapeutic Outcome of B-Acute Lymphoblastic Leukemia Patients.
Baba SM, Pandith AA, Shah ZA, Geelani SA, Bhat JR, Gul A, Guru SA, El-Serehy HA, Koul AM, Mansoor S. Baba SM, et al. Front Oncol. 2021 Oct 14;11:714421. doi: 10.3389/fonc.2021.714421. eCollection 2021. Front Oncol. 2021. PMID: 34722260 Free PMC article. - HCV infection causes cirrhosis in human by step-wise regulation of host genes involved in cellular functioning and defense during fibrosis: Identification of bio-markers.
Ijaz B, Ahmad W, Das T, Shabbiri K, Husnain T, Hassan S. Ijaz B, et al. Genes Dis. 2019 May 8;6(3):304-317. doi: 10.1016/j.gendis.2019.04.007. eCollection 2019 Sep. Genes Dis. 2019. PMID: 32042870 Free PMC article. - Association of GSTO1 A140D and GSTO2 N142D Gene Variations with Breast Cancer Risk.
Sharif MR, Sharif A, Kheirkhah D, Taghavi Ardakan M, Soltani N. Sharif MR, et al. Asian Pac J Cancer Prev. 2017 Jun 25;18(6):1723-1727. doi: 10.22034/APJCP.2017.18.6.1723. Asian Pac J Cancer Prev. 2017. PMID: 28670895 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous