Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP - PubMed (original) (raw)

Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP

G J Cote et al. Nucleic Acids Res. 1992.

Free PMC article

Abstract

Transcripts derived from the 6 exon CALC I gene are differentially processed in a tissue-specific fashion to include or exclude a calcitonin-specific exon 4. All cell types which transcribe a second calcitonin/CGRP gene, CALC II, exclude exon 4. Substitution of the first 30 nucleotides of CALC I exon 4 with analogous CALC II sequence was sufficient to prevent recognition of exon 4 in in vitro or in vivo RNA splicing systems. UV crosslinking detected a approximately 66 kDa RNA-binding protein in HeLa nuclear extract which interacted with CALC I proximal exon sequence, but not CALC II or mutant sequences. UV crosslinking of this protein was inhibited by addition of nuclear extract from a cell type which normally causes exclusion of exon 4. These results identify an important regulatory element within exon 4 and support a model in which calcitonin production requires protein interaction with this sequence to facilitate exon recognition.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nucleic Acids Res. 1991 Jul 11;19(13):3601-6 - PubMed
    1. Cell. 1991 May 17;65(4):579-86 - PubMed
    1. Science. 1991 May 10;252(5007):833-6 - PubMed
    1. Genes Dev. 1991 May;5(5):786-96 - PubMed
    1. Mol Endocrinol. 1990 Nov;4(11):1744-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources