The respiratory pathogen Moraxella catarrhalis adheres to epithelial cells by interacting with fibronectin through ubiquitous surface proteins A1 and A2 - PubMed (original) (raw)

. 2005 Sep 15;192(6):1029-38.

doi: 10.1086/432759. Epub 2005 Aug 12.

Affiliations

• PMID: 16107956
• DOI: 10.1086/432759

The respiratory pathogen Moraxella catarrhalis adheres to epithelial cells by interacting with fibronectin through ubiquitous surface proteins A1 and A2

Thuan Tong Tan et al. J Infect Dis. 2005.

Abstract

Moraxella catarrhalis ubiquitous surface protein (Usp) A1 has been reported to bind fibronectin and is involved in adherence. In this study, using M. catarrhalis mutants derived from clinical isolates, we show that both UspA1 and UspA2 bind fibronectin. Recombinant truncated UspA1/A2 proteins, together with smaller fragments spanning the entire molecule, were tested for binding to fibronectin. Both UspA1 and UspA2 bound fibronectin, and the fibronectin-binding domains were located within UspA1(299-452) and UspA2(165-318). These 2 truncated proteins inhibited binding of M. catarrhalis to Chang conjunctival epithelial cells to an extent similar to that by anti-human fibronectin antibodies. Our observations show that both UspA1 and UspA2 are involved in adherence to epithelial cells via cell-associated fibronectin. The biologically active sites within UspA1(299-452) and UspA2(165-318) have therefore been suggested to be potential candidates to be included in a future vaccine against M. catarrhalis.

PubMed Disclaimer

Similar articles

• The respiratory pathogen moraxella catarrhalis binds to laminin via ubiquitous surface proteins A1 and A2.
  Tan TT, Forsgren A, Riesbeck K. Tan TT, et al. J Infect Dis. 2006 Aug 15;194(4):493-7. doi: 10.1086/505581. Epub 2006 Jul 7. J Infect Dis. 2006. PMID: 16845633
• MID and UspA1/A2 of the human respiratory pathogen Moraxella catarrhalis, and interactions with the human host as basis for vaccine development.
  Riesbeck K, Tan TT, Forsgren A. Riesbeck K, et al. Acta Biochim Pol. 2006;53(3):445-56. Epub 2006 Sep 9. Acta Biochim Pol. 2006. PMID: 16964325 Review.
• The emerging pathogen Moraxella catarrhalis interacts with complement inhibitor C4b binding protein through ubiquitous surface proteins A1 and A2.
  Nordström T, Blom AM, Forsgren A, Riesbeck K. Nordström T, et al. J Immunol. 2004 Oct 1;173(7):4598-606. doi: 10.4049/jimmunol.173.7.4598. J Immunol. 2004. PMID: 15383594
• uspA1 of Moraxella catarrhalis clinical isolates in Japan and its relationship with adherence to HEp-2 cells.
  Qiu H, Kumita W, Sato K, Nakajima S, Nishiyama H, Saito R, Sawabe E, Ono E, Chida T, Okamura N. Qiu H, et al. J Med Dent Sci. 2009 Mar;56(1):61-7. J Med Dent Sci. 2009. PMID: 19697520
• Current progress of adhesins as vaccine candidates for Moraxella catarrhalis.
  Tan TT, Riesbeck K. Tan TT, et al. Expert Rev Vaccines. 2007 Dec;6(6):949-56. doi: 10.1586/14760584.6.6.949. Expert Rev Vaccines. 2007. PMID: 18377357 Review.

Cited by

• Rhinovirus increases Moraxella catarrhalis adhesion to the respiratory epithelium.
  Dissanayake E, Brockman-Schneider RA, Stubbendieck RM, Helling BA, Zhang Z, Bochkov YA, Kirkham C, Murphy TF, Ober C, Currie CR, Gern JE. Dissanayake E, et al. Front Cell Infect Microbiol. 2023 Jan 17;12:1060748. doi: 10.3389/fcimb.2022.1060748. eCollection 2022. Front Cell Infect Microbiol. 2023. PMID: 36733852 Free PMC article.
• Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells.
  Liu YL, Ding R, Jia XM, Huang JJ, Yu S, Chan HT, Li W, Mao LL, Zhang L, Zhang XY, Wu W, Ni AP, Xu YC. Liu YL, et al. Emerg Microbes Infect. 2022 Dec;11(1):2055-2068. doi: 10.1080/22221751.2022.2108341. Emerg Microbes Infect. 2022. PMID: 35904140 Free PMC article.
• Interaction of Bartonella henselae with Fibronectin Represents the Molecular Basis for Adhesion to Host Cells.
  Vaca DJ, Thibau A, Leisegang MS, Malmström J, Linke D, Eble JA, Ballhorn W, Schaller M, Happonen L, Kempf VAJ. Vaca DJ, et al. Microbiol Spectr. 2022 Jun 29;10(3):e0059822. doi: 10.1128/spectrum.00598-22. Epub 2022 Apr 18. Microbiol Spectr. 2022. PMID: 35435766 Free PMC article.
• Interactions of Bacteriophages and Bacteria at the Airway Mucosa: New Insights Into the Pathophysiology of Asthma.
  Tzani-Tzanopoulou P, Skliros D, Megremis S, Xepapadaki P, Andreakos E, Chanishvili N, Flemetakis E, Kaltsas G, Taka S, Lebessi E, Doudoulakakis A, Papadopoulos NG. Tzani-Tzanopoulou P, et al. Front Allergy. 2021 Jan 26;1:617240. doi: 10.3389/falgy.2020.617240. eCollection 2020. Front Allergy. 2021. PMID: 35386933 Free PMC article. Review.
• CEACAM1 Activation by CbpF-Expressing E. coli.
  Shhadeh A, Galaski J, Alon-Maimon T, Fahoum J, Wiener R, Slade DJ, Mandelboim O, Bachrach G. Shhadeh A, et al. Front Cell Infect Microbiol. 2021 Jul 29;11:699015. doi: 10.3389/fcimb.2021.699015. eCollection 2021. Front Cell Infect Microbiol. 2021. PMID: 34395310 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations