Direct isolation of satellite cells for skeletal muscle regeneration - PubMed (original) (raw)
. 2005 Sep 23;309(5743):2064-7.
doi: 10.1126/science.1114758. Epub 2005 Sep 1.
Affiliations
- PMID: 16141372
- DOI: 10.1126/science.1114758
Direct isolation of satellite cells for skeletal muscle regeneration
Didier Montarras et al. Science. 2005.
Abstract
Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
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