The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity - PubMed (original) (raw)
doi: 10.1038/nsmb990. Epub 2005 Sep 18.
Affiliations
- PMID: 16170325
- DOI: 10.1038/nsmb990
The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity
Lionel Ballut et al. Nat Struct Mol Biol. 2005 Oct.
Abstract
The multiprotein exon junction complex (EJC) is assembled on mRNAs as a consequence of splicing. EJC core components maintain a stable grip on mRNAs even as the overall EJC protein composition evolves while mRNAs travel to the cytoplasm. Here we show that recombinant EJC subunits MLN51, MAGOH and Y14, together with the DEAD-box protein eIF4AIII bound to ATP, are necessary and sufficient to form a highly stable complex on single-stranded RNA. Cross-linking and RNase protection studies indicate that this recombinant complex recapitulates the EJC core. The stable association of the recombinant EJC core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. We elucidate the modalities of EJC binding to RNA and provide the first example of how cellular machineries may use RNA helicases to clamp several proteins onto RNA in stable and sequence-independent manners.
Similar articles
- Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR. Andersen CB, et al. Science. 2006 Sep 29;313(5795):1968-72. doi: 10.1126/science.1131981. Epub 2006 Aug 24. Science. 2006. PMID: 16931718 - The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA.
Bono F, Ebert J, Lorentzen E, Conti E. Bono F, et al. Cell. 2006 Aug 25;126(4):713-25. doi: 10.1016/j.cell.2006.08.006. Cell. 2006. PMID: 16923391 - MLN51 stimulates the RNA-helicase activity of eIF4AIII.
Noble CG, Song H. Noble CG, et al. PLoS One. 2007 Mar 21;2(3):e303. doi: 10.1371/journal.pone.0000303. PLoS One. 2007. PMID: 17375189 Free PMC article. - Structural insights into the exon junction complex.
Le Hir H, Andersen GR. Le Hir H, et al. Curr Opin Struct Biol. 2008 Feb;18(1):112-9. doi: 10.1016/j.sbi.2007.11.002. Curr Opin Struct Biol. 2008. PMID: 18164611 Review. - The Physiological Roles of the Exon Junction Complex in Development and Diseases.
Asthana S, Martin H, Rupkey J, Patel S, Yoon J, Keegan A, Mao Y. Asthana S, et al. Cells. 2022 Apr 1;11(7):1192. doi: 10.3390/cells11071192. Cells. 2022. PMID: 35406756 Free PMC article. Review.
Cited by
- DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans.
Longman D, Hug N, Keith M, Anastasaki C, Patton EE, Grimes G, Cáceres JF. Longman D, et al. Nucleic Acids Res. 2013 Sep;41(17):8319-31. doi: 10.1093/nar/gkt585. Epub 2013 Jul 4. Nucleic Acids Res. 2013. PMID: 23828042 Free PMC article. - DEAD-box helicases as integrators of RNA, nucleotide and protein binding.
Putnam AA, Jankowsky E. Putnam AA, et al. Biochim Biophys Acta. 2013 Aug;1829(8):884-93. doi: 10.1016/j.bbagrm.2013.02.002. Epub 2013 Feb 15. Biochim Biophys Acta. 2013. PMID: 23416748 Free PMC article. Review. - Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. Barbosa I, et al. Nat Struct Mol Biol. 2012 Oct;19(10):983-90. doi: 10.1038/nsmb.2380. Epub 2012 Sep 9. Nat Struct Mol Biol. 2012. PMID: 22961380 - Substrate-assisted mechanism of RNP disruption by the spliceosomal Brr2 RNA helicase.
Theuser M, Höbartner C, Wahl MC, Santos KF. Theuser M, et al. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7798-803. doi: 10.1073/pnas.1524616113. Epub 2016 Jun 27. Proc Natl Acad Sci U S A. 2016. PMID: 27354531 Free PMC article. - Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.
Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, Garneau D, Gendron D, Lapointe E, Couture S, Le Hir H, Klinck R, Elela SA, Prinos P, Chabot B. Michelle L, et al. Mol Cell Biol. 2012 Mar;32(5):954-67. doi: 10.1128/MCB.06130-11. Epub 2011 Dec 27. Mol Cell Biol. 2012. PMID: 22203037 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases