IgE-dependent activation of sphingosine kinases 1 and 2 and secretion of sphingosine 1-phosphate requires Fyn kinase and contributes to mast cell responses - PubMed (original) (raw)
. 2006 Feb 3;281(5):2515-25.
doi: 10.1074/jbc.M508931200. Epub 2005 Nov 29.
Affiliations
- PMID: 16316995
- DOI: 10.1074/jbc.M508931200
Free article
IgE-dependent activation of sphingosine kinases 1 and 2 and secretion of sphingosine 1-phosphate requires Fyn kinase and contributes to mast cell responses
Ana Olivera et al. J Biol Chem. 2006.
Free article
Abstract
Engagement of the high affinity receptor for IgE (FcepsilonRI) on mast cells results in the production and secretion of sphingosine 1-phosphate (S1P), a lipid metabolite present in the lungs of allergen-challenged asthmatics. Herein we report that two isoforms of sphingosine kinase (SphK1 and SphK2) are expressed and activated upon FcepsilonRI engagement of bone marrow-derived mast cells (BMMC). Fyn kinase is required for FcepsilonRI coupling to SphK1 and -2 and for subsequent S1P production. Normal activation of SphK1 and -2 was restored by expression of wild type Fyn but only partly with a kinase-defective Fyn, indicating that induction of SphK1 and SphK2 depended on both catalytic and noncatalytic properties of Fyn. Downstream of Fyn, the requirements for SphK1 activation differed from that of SphK2. Whereas SphK1 was considerably dependent on the adapter Grb2-associated binder 2 and phosphatidylinositol 3-OH kinase, SphK2 showed minimal dependence on these molecules. Fyn-deficient BMMC were defective in chemotaxis and, as previously reported, in degranulation. These functional responses were partly reconstituted by the addition of exogenous S1P to FcepsilonRI-stimulated cells. Taken together with our previous study, which demonstrated delayed SphK activation in Lyn-deficient BMMC, we propose a cooperative role between Fyn and Lyn kinases in the activation of SphKs, which contributes to mast cell responses.
Similar articles
- Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined.
Dillahunt SE, Sargent JL, Suzuki R, Proia RL, Gilfillan A, Rivera J, Olivera A. Dillahunt SE, et al. J Immunol. 2013 Mar 1;190(5):2058-67. doi: 10.4049/jimmunol.1201503. Epub 2013 Jan 28. J Immunol. 2013. PMID: 23359503 Free PMC article. - Early activation of sphingosine kinase in mast cells and recruitment to FcepsilonRI are mediated by its interaction with Lyn kinase.
Urtz N, Olivera A, Bofill-Cardona E, Csonga R, Billich A, Mechtcheriakova D, Bornancin F, Woisetschläger M, Rivera J, Baumruker T. Urtz N, et al. Mol Cell Biol. 2004 Oct;24(19):8765-77. doi: 10.1128/MCB.24.19.8765-8777.2004. Mol Cell Biol. 2004. PMID: 15367693 Free PMC article. - Transactivation of sphingosine-1-phosphate receptors by FcepsilonRI triggering is required for normal mast cell degranulation and chemotaxis.
Jolly PS, Bektas M, Olivera A, Gonzalez-Espinosa C, Proia RL, Rivera J, Milstien S, Spiegel S. Jolly PS, et al. J Exp Med. 2004 Apr 5;199(7):959-70. doi: 10.1084/jem.20030680. J Exp Med. 2004. PMID: 15067032 Free PMC article. - Src family kinases and lipid mediators in control of allergic inflammation.
Rivera J, Olivera A. Rivera J, et al. Immunol Rev. 2007 Jun;217:255-68. doi: 10.1111/j.1600-065X.2007.00505.x. Immunol Rev. 2007. PMID: 17498064 Review. - A current understanding of Fc epsilon RI-dependent mast cell activation.
Rivera J, Olivera A. Rivera J, et al. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20. doi: 10.1007/s11882-008-0004-z. Curr Allergy Asthma Rep. 2008. PMID: 18377769 Review.
Cited by
- Sphingosine-1-phosphate can promote mast cell hyper-reactivity through regulation of contactin-4 expression.
Olivera A, Kitamura Y, Wright LD, Allende ML, Chen W, Kaneko-Goto T, Yoshihara Y, Proia RL, Rivera J. Olivera A, et al. J Leukoc Biol. 2013 Nov;94(5):1013-24. doi: 10.1189/jlb.0313163. Epub 2013 Jul 31. J Leukoc Biol. 2013. PMID: 23904439 Free PMC article. - The immunosuppressant drug FTY720 inhibits cytosolic phospholipase A2 independently of sphingosine-1-phosphate receptors.
Payne SG, Oskeritzian CA, Griffiths R, Subramanian P, Barbour SE, Chalfant CE, Milstien S, Spiegel S. Payne SG, et al. Blood. 2007 Feb 1;109(3):1077-85. doi: 10.1182/blood-2006-03-011437. Epub 2006 Sep 28. Blood. 2007. PMID: 17008548 Free PMC article. - Molecular mechanisms of regulation of sphingosine kinase 1.
Pulkoski-Gross MJ, Obeid LM. Pulkoski-Gross MJ, et al. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Nov;1863(11):1413-1422. doi: 10.1016/j.bbalip.2018.08.015. Epub 2018 Aug 30. Biochim Biophys Acta Mol Cell Biol Lipids. 2018. PMID: 30591148 Free PMC article. Review. - Altered Sphingolipid Metabolism Is Associated With Asthma Phenotype in House Dust Mite-Allergic Patients.
Kowal K, Żebrowska E, Chabowski A. Kowal K, et al. Allergy Asthma Immunol Res. 2019 May;11(3):330-342. doi: 10.4168/aair.2019.11.3.330. Allergy Asthma Immunol Res. 2019. PMID: 30912323 Free PMC article. - Aryl hydrocarbon receptor signaling promotes ORMDL3-dependent generation of sphingosine-1-phosphate by inhibiting sphingosine-1-phosphate lyase.
Wang HC, Wong TH, Wang LT, Su HH, Yu HY, Wu AH, Lin YC, Chen HL, Suen JL, Hsu SH, Chen LC, Zhou Y, Huang SK. Wang HC, et al. Cell Mol Immunol. 2019 Oct;16(10):783-790. doi: 10.1038/s41423-018-0022-2. Epub 2018 Mar 23. Cell Mol Immunol. 2019. PMID: 29572542 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous