Germinal and somatic products of Mu1 excision from the Bronze-1 gene of Zea mays - PubMed (original) (raw)

. 1991 Jun;227(2):267-76.

doi: 10.1007/BF00259680.

Affiliations

Germinal and somatic products of Mu1 excision from the Bronze-1 gene of Zea mays

A B Britt et al. Mol Gen Genet. 1991 Jun.

Abstract

Germinal and somatic excision products of Mu1 from the insertion allele bz::mu1 were selectively amplified from maize cob tissue. The sequence of these "footprints" often included deletions at the target site, suggesting that substantial exonucleolytic degradation occurs upon excision of the element. In addition to deletions of target site sequences, single base insertions were also found. The isolation of an excision product including a 4 bp inverted duplication of the target site provides evidence that the double-stranded chromosomal break generated by Mu excision may be terminated by a covalently closed hairpin structure. The majority of excision products, however, do not include inverted duplications of target site sequences, suggesting that such structures are the result of occasional repair activities, rather than an essential step in the mechanism of Mu excision. The sequence of the Mu insertion sites of the bz::mu1 and bz::mu2 alleles is also presented.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Plant Cell. 1990 Dec;2(12):1225-38 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9451-5 - PubMed
    1. Genetics. 1948 May;33(3):273-303 - PubMed
    1. Mol Gen Genet. 1988 Jan;211(1):27-34 - PubMed
    1. J Mol Evol. 1989 Jul;29(1):28-39 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources