Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans - PubMed (original) (raw)
Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans
K H Fritzemeier et al. Plant Physiol. 1987 Sep.
Abstract
Infection of potato leaves with the fungal pathogen Phytophthora infestans (Pi) resulted in the rapid stimulation of phenylpropanoid metabolism. Increases in the activities of several mRNAs, including those encoding phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL), were detectable within a few hours postinoculation, as demonstrated by two-dimensional gel electrophoresis of proteins synthesized in vitro. This effect was closely mimicked by application of Pi culture filtrate through cut leaf stems. PAL and 4CL mRNA activities were also rapidly and transiently induced in potato cell suspension cultures by treatments with Pi culture filtrate or arachidonic acid. This induction was exploited to generate cDNA probes complementary to PAL and 4CL mRNAs. Blot hybridizations using these probes revealed almost immediate, transient and coordinate increases in the transcription rates and subsequent changes in the amounts of PAL and 4CL mRNAs in leaves treated with Pi culture filtrate. Similar changes in the mRNA amounts were found in infected leaves of potato cultivars carrying resistance genes R1 (cv Datura) or R4 (cv Isola), independent of whether a virulent or an avirulent Pi pathotype was used for inoculation. These results are discussed in relation to recent cytological observations with the same potato cultivars and Pi pathotypes.
Similar articles
- Induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor.
Kuhn DN, Chappell J, Boudet A, Hahlbrock K. Kuhn DN, et al. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1102-6. doi: 10.1073/pnas.81.4.1102. Proc Natl Acad Sci U S A. 1984. PMID: 16593418 Free PMC article. - Differential wound activation of members of the phenylalanine ammonia-lyase and 4-coumarate:CoA ligase gene families in various organs of parsley plants.
Lois R, Hahlbrock K. Lois R, et al. Z Naturforsch C J Biosci. 1992 Jan-Feb;47(1-2):90-4. doi: 10.1515/znc-1992-1-216. Z Naturforsch C J Biosci. 1992. PMID: 1376996 - Phenylalanine ammonia-lyase in potato (Solanum tuberosum L.). Genomic complexity, structural comparison of two selected genes and modes of expression.
Joos HJ, Hahlbrock K. Joos HJ, et al. Eur J Biochem. 1992 Mar 1;204(2):621-9. doi: 10.1111/j.1432-1033.1992.tb16675.x. Eur J Biochem. 1992. PMID: 1541277 - Modes of expression and common structural features of the complete phenylalanine ammonia-lyase gene family in parsley.
Logemann E, Parniske M, Hahlbrock K. Logemann E, et al. Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5905-9. doi: 10.1073/pnas.92.13.5905. Proc Natl Acad Sci U S A. 1995. PMID: 7597051 Free PMC article. - Rapid Response of Suspension-cultured Parsley Cells to the Elicitor from Phytophthora megasperma var. sojae: INDUCTION OF THE ENZYMES OF GENERAL PHENYLPROPANOID METABOLISM.
Hahlbrock K, Lamb CJ, Purwin C, Ebel J, Fautz E, Schäfer E. Hahlbrock K, et al. Plant Physiol. 1981 Apr;67(4):768-73. doi: 10.1104/pp.67.4.768. Plant Physiol. 1981. PMID: 16661752 Free PMC article.
Cited by
- Genome-Wide Identification and Expression Analysis of the 4-Coumarate: CoA Ligase Gene Family in Solanum tuberosum.
Nie T, Sun X, Wang S, Wang D, Ren Y, Chen Q. Nie T, et al. Int J Mol Sci. 2023 Jan 13;24(2):1642. doi: 10.3390/ijms24021642. Int J Mol Sci. 2023. PMID: 36675157 Free PMC article. - The protein phosphatase 2A catalytic subunit StPP2Ac2b enhances susceptibility to Phytophthora infestans and senescence in potato.
Muñiz García MN, Grossi C, Ulloa RM, Capiati DA. Muñiz García MN, et al. PLoS One. 2022 Oct 10;17(10):e0275844. doi: 10.1371/journal.pone.0275844. eCollection 2022. PLoS One. 2022. PMID: 36215282 Free PMC article. - Genome-Wide Identification and Gene Expression Analysis of Acyl-Activating Enzymes Superfamily in Tomato (Solanum lycopersicum) Under Aluminum Stress.
Jin JF, He QY, Li PF, Lou HQ, Chen WW, Yang JL. Jin JF, et al. Front Plant Sci. 2021 Dec 2;12:754147. doi: 10.3389/fpls.2021.754147. eCollection 2021. Front Plant Sci. 2021. PMID: 34925406 Free PMC article. - Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease.
Xiao C, Huang M, Gao J, Wang Z, Zhang D, Zhang Y, Yan L, Yu X, Li B, Shen Y. Xiao C, et al. BMC Genomics. 2020 Dec 9;21(1):880. doi: 10.1186/s12864-020-07286-3. BMC Genomics. 2020. PMID: 33297944 Free PMC article. - Structural, functional and evolutionary diversity of 4-coumarate-CoA ligase in plants.
Lavhale SG, Kalunke RM, Giri AP. Lavhale SG, et al. Planta. 2018 Nov;248(5):1063-1078. doi: 10.1007/s00425-018-2965-z. Epub 2018 Aug 4. Planta. 2018. PMID: 30078075 Review.
References
- Science. 1978 Jul 28;201(4353):364-5 - PubMed
- Gene. 1983 Nov;25(2-3):263-9 - PubMed
- Anal Biochem. 1976 May 7;72:248-54 - PubMed
- Science. 1977 Apr 8;196(4286):180-2 - PubMed
- Proc Natl Acad Sci U S A. 1984 Feb;81(4):1102-6 - PubMed
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous