Caveolin-1 is required for fatty acid translocase (FAT/CD36) localization and function at the plasma membrane of mouse embryonic fibroblasts - PubMed (original) (raw)

. 2006 Apr;1761(4):416-23.

doi: 10.1016/j.bbalip.2006.03.016. Epub 2006 Apr 19.

Affiliations

• PMID: 16702023
• DOI: 10.1016/j.bbalip.2006.03.016

Free article

Caveolin-1 is required for fatty acid translocase (FAT/CD36) localization and function at the plasma membrane of mouse embryonic fibroblasts

Axel Ring et al. Biochim Biophys Acta. 2006 Apr.

Free article

Abstract

Several lines of evidence suggest that lipid rafts are involved in cellular fatty acid uptake and influence fatty acid translocase (FAT/CD36) function. However, it remains unknown whether caveolae, a specialized raft type, are required for this mechanism. Here, we show that wild-type (WT) mouse embryonic fibroblasts (MEFs) and caveolin-1 knockout (KO) MEFs, which are devoid of caveolae, have comparable overall expression of FAT/CD36 protein but altered subcellular FAT/CD36 localization and function. In WT MEFs, FAT/CD36 was isolated with both lipid raft enriched detergent-resistant membranes (DRMs) and detergent-soluble membranes (DSMs), whereas in cav-1 KO cells it was exclusively associated with DSMs. Subcellular fractionation demonstrated that FAT/CD36 in WT MEFs was localized intracellularly and at the plasma membrane level while in cav-1 KO MEFs it was absent from the plasma membrane. This mistargeting of FAT/CD36 in cav-1 KO cells resulted in reduced fatty acid uptake compared to WT controls. Adenoviral expression of caveolin-1 in KO MEFs induced caveolae formation, redirection of FAT/CD36 to the plasma membrane and rescue of fatty acid uptake. In conclusion, our data provide evidence that caveolin-1 is necessary to target FAT/CD36 to the plasma membrane. Caveolin-1 may influence fatty acid uptake by regulating surface availability of FAT/CD36.

PubMed Disclaimer

Similar articles

• FAT/CD36-mediated long-chain fatty acid uptake in adipocytes requires plasma membrane rafts.
  Pohl J, Ring A, Korkmaz U, Ehehalt R, Stremmel W. Pohl J, et al. Mol Biol Cell. 2005 Jan;16(1):24-31. doi: 10.1091/mbc.e04-07-0616. Epub 2004 Oct 20. Mol Biol Cell. 2005. PMID: 15496455 Free PMC article.
• FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate.
  Eyre NS, Cleland LG, Mayrhofer G. Eyre NS, et al. Biochem Biophys Res Commun. 2008 Jun 6;370(3):404-9. doi: 10.1016/j.bbrc.2008.02.164. Epub 2008 Mar 24. Biochem Biophys Res Commun. 2008. PMID: 18364236
• Long-chain fatty acid uptake into adipocytes depends on lipid raft function.
  Pohl J, Ring A, Ehehalt R, Schulze-Bergkamen H, Schad A, Verkade P, Stremmel W. Pohl J, et al. Biochemistry. 2004 Apr 13;43(14):4179-87. doi: 10.1021/bi035743m. Biochemistry. 2004. PMID: 15065861
• Sulfo-N-succinimidyl esters of long chain fatty acids specifically inhibit fatty acid translocase (FAT/CD36)-mediated cellular fatty acid uptake.
  Coort SL, Willems J, Coumans WA, van der Vusse GJ, Bonen A, Glatz JF, Luiken JJ. Coort SL, et al. Mol Cell Biochem. 2002 Oct;239(1-2):213-9. Mol Cell Biochem. 2002. PMID: 12479588 Review.
• From fat to FAT (CD36/SR-B2): Understanding the regulation of cellular fatty acid uptake.
  Glatz JF, Luiken JJ. Glatz JF, et al. Biochimie. 2017 May;136:21-26. doi: 10.1016/j.biochi.2016.12.007. Epub 2016 Dec 22. Biochimie. 2017. PMID: 28013071 Review.

Cited by

• Endothelial cell CD36 regulates membrane ceramide formation, exosome fatty acid transfer and circulating fatty acid levels.
  Peche VS, Pietka TA, Jacome-Sosa M, Samovski D, Palacios H, Chatterjee-Basu G, Dudley AC, Beatty W, Meyer GA, Goldberg IJ, Abumrad NA. Peche VS, et al. Nat Commun. 2023 Jul 7;14(1):4029. doi: 10.1038/s41467-023-39752-3. Nat Commun. 2023. PMID: 37419919 Free PMC article.
• CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis.
  Hao JW, Wang J, Guo H, Zhao YY, Sun HH, Li YF, Lai XY, Zhao N, Wang X, Xie C, Hong L, Huang X, Wang HR, Li CB, Liang B, Chen S, Zhao TJ. Hao JW, et al. Nat Commun. 2020 Sep 21;11(1):4765. doi: 10.1038/s41467-020-18565-8. Nat Commun. 2020. PMID: 32958780 Free PMC article.
• Coplanar polychlorinated biphenyl-induced CYP1A1 is regulated through caveolae signaling in vascular endothelial cells.
  Lim EJ, Májková Z, Xu S, Bachas L, Arzuaga X, Smart E, Tseng MT, Toborek M, Hennig B. Lim EJ, et al. Chem Biol Interact. 2008 Nov 25;176(2-3):71-8. doi: 10.1016/j.cbi.2008.08.007. Epub 2008 Aug 22. Chem Biol Interact. 2008. PMID: 18786521 Free PMC article.
• Hepatic fatty acid uptake is regulated by the sphingolipid acyl chain length.
  Park WJ, Park JW, Merrill AH, Storch J, Pewzner-Jung Y, Futerman AH. Park WJ, et al. Biochim Biophys Acta. 2014 Dec;1841(12):1754-66. doi: 10.1016/j.bbalip.2014.09.009. Biochim Biophys Acta. 2014. PMID: 25241943 Free PMC article.
• Lipid droplet analysis in caveolin-deficient adipocytes: alterations in surface phospholipid composition and maturation defects.
  Blouin CM, Le Lay S, Eberl A, Köfeler HC, Guerrera IC, Klein C, Le Liepvre X, Lasnier F, Bourron O, Gautier JF, Ferré P, Hajduch E, Dugail I. Blouin CM, et al. J Lipid Res. 2010 May;51(5):945-56. doi: 10.1194/jlr.M001016. Epub 2009 Nov 10. J Lipid Res. 2010. PMID: 19965594 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program