Central and medial amygdaloid brain-derived neurotrophic factor signaling plays a critical role in alcohol-drinking and anxiety-like behaviors - PubMed (original) (raw)

Central and medial amygdaloid brain-derived neurotrophic factor signaling plays a critical role in alcohol-drinking and anxiety-like behaviors

Subhash C Pandey et al. J Neurosci. 2006.

Erratum in

Abstract

Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family of neurotrophins and plays a vital role in synaptic plasticity. This study investigated the involvement of the amygdaloid BDNF system in molecular mechanisms underlying anxiety and alcohol-drinking behaviors. Male Sprague Dawley rats were cannulated targeting central amygdala (CeA), medial amygdala (MeA), or basolateral amygdala (BLA), and BDNF expression was manipulated using an antisense oligodeoxynucleotide (ODN) strategy. Anxiety-like and alcohol-drinking behaviors were measured after infusion of BDNF sense and antisense ODNs with or without BDNF coinfusion, using the elevated plus-maze test and two-bottle free-choice paradigm, respectively. Here we report that BDNF antisense ODN infusions into the CeA and MeA, but not BLA, provoked anxiety-like behaviors in rats, which were rescued by BDNF coinfusion. The levels of BDNF, p-ERK1/2 (phosphorylated extracellular signal-regulated kinases 1/2), and p-CREB (phosphorylated cAMP responsive-element binding protein) were decreased by BDNF antisense, but not by sense, ODN infusions, which were restored to normal after BDNF coinfusions. Furthermore, BDNF antisense ODN infusions into the CeA or MeA, but not into BLA, increased alcohol intake, which was attenuated by BDNF coinfusions. These novel results suggest that decreased BDNF levels in the CeA and MeA, but not in the BLA, are crucial in regulating alcohol-drinking and anxiety-like behaviors in rats.

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Figures

Figure 1.

Figure 1.

A, Low-magnification view of Nissl staining of the amygdaloid structures of rats. This photomicrograph indicates the position of the cannula, which is just above the targeted area of central amygdala. Scale bar, 200 μm. B, Effect of CeA infusion of aCSF and BDNF sense and antisense ODNs (with and without BDNF coinfusion) on open- and closed-arm activities of the EPM. Total number of entries represents the total numbers of closed- and open-arm entries in EPM. Values are the mean ± SEM of eight rats in each group. Asterisks indicate significant (p < 0.01–0.001) difference from aCSF-infused rats.

Figure 2.

Figure 2.

Representative photomicrographs at low magnification showing BDNF (top row), p-CREB (third row), p-ERK1/2 (fourth row), and NeuN (bottom row) gold-immunolabeling and mRNA levels of BDNF (second row) in the CeA brain structures of rats infused with aCSF, BDNF sense, and antisense ODNs (with and without BDNF coinfusion) into the CeA. Scale bar, 30 μm. Insets in the aCSF column indicate immunogold particles within a single cell body or single nucleus at high magnification (100×).

Figure 3.

Figure 3.

Quantitation of BDNF, p-CREB, and p-ERK1/2 protein levels (number of immunogold particles per 100 μm2 area) and mRNA levels of BDNF in the amygdaloid structures of rats infused with aCSF, BDNF sense, and antisense ODNs (with and without BDNF coinfusion) into the CeA. Values are the mean ± SEM of five to seven rats in each group. The asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 4.

Figure 4.

Effect of CeA infusion (once at day 1) of BDNF sense and antisense ODNs and/or BDNF (twice; first infusion at day 1 and second infusion at day 2) on alcohol preference as measured by the two-bottle free-choice paradigm. Results represent the percentage of 7% v/v (ethanol in water solution) ethanol intake and percentage of water intake of total fluid intake (milliliters per day). Values are the mean ± SEM of six rats in each group, and alcohol intake was measured for 4 d in the same group of rats. Asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 5.

Figure 5.

A, Low-magnification view of Nissl staining of the amygdaloid structures of rats. This photomicrograph indicates the position of the cannula, which is just above the targeted area of medial amygdala. Scale bar, 200 μm. B, Effect of MeA infusion of aCSF and BDNF sense and antisense ODNs (with and without BDNF coinfusion) on open- and closed-arm activities of the elevated plus maze. Total number of entries represents the total numbers of closed- and open-arm entries in EPM. Values are the mean ± SEM of eight to nine rats in each group. Asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 6.

Figure 6.

Representative photomicrographs at low magnification showing BDNF (top row), p-CREB (third row), p-ERK1/2 (fourth row), and NeuN (bottom row) gold immunolabeling and mRNA levels of BDNF (second row) in the MeA brain structures of rats infused with aCSF, BDNF sense, and antisense ODNs (with and without BDNF coinfusion) into the MeA. Scale bar, 30 μm. Insets in the aCSF column indicate immunogold particles within a single cell body or single nucleus at high magnification (100×).

Figure 7.

Figure 7.

Quantitation of BDNF, p-CREB, and p-ERK1/2 protein levels (number of immunogold particles per 100 μm2 area) and mRNA levels of BDNF in the amygdaloid structures of rats infused in the MeA with aCSF and BDNF sense and antisense ODNs (with and without BDNF coinfusion). Values are the mean ± SEM of five to seven rats in each group. Asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 8.

Figure 8.

Effect of MeA infusion (once at day 1) of BDNF sense and antisense ODNs and/or BDNF (twice; first infusion at day 1 and second infusion at day 2) on alcohol preference as measured by the two-bottle free-choice paradigm. Results represent the percentage of 7% v/v (ethanol in water solution) ethanol intake and percentage of water intake of total fluid intake (milliliters per day). Values are the mean ± SEM of six rats in each group, and alcohol intake was measured for 4 d in the same group of rats. Asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 9.

Figure 9.

A, Low-magnification view of Nissl staining of amygdaloid structures of rats. This photomicrograph indicates the position of the cannula, which is just above the targeted area of basolateral amygdala. Scale bar, 200 μm. B, Effect of BLA infusion of aCSF and BDNF sense and antisense ODNs (with and without BDNF) on open- and closed-arm activities of the elevated plus maze. Total number of entries represents the total numbers of closed- and open-arm entries in EPM. Values are the mean ± SEM of 6–12 rats in each group.

Figure 10.

Figure 10.

Representative photomicrographs at low magnification showing BDNF (top row), p-CREB (third row), p-ERK1/2 (fourth row), and NeuN (bottom row) gold immunolabeling and mRNA levels of BDNF (second row) in the BLA brain structures of rats infused with aCSF and BDNF sense and antisense ODNs (with or without BDNF infusion) into the BLA. Scale bar, 30 μm. Insets in the aCSF column indicate immunogold particles within a single cell body or single nucleus at high magnification (100×).

Figure 11.

Figure 11.

Quantitation of BDNF, p-CREB, and p-ERK1/2 protein levels (number of immunogold particles per 100 μm2 area) and mRNA levels of BDNF in the amygdaloid structures of rats infused in the BLA with aCSF and BDNF sense and antisense ODNs (with or without BDNF coinfusion). Values are the mean ± SEM of 6–12 rats in each group. Asterisks indicate significant (p < 0.001) difference from aCSF-infused rats.

Figure 12.

Figure 12.

Effect of BLA infusion of BDNF sense and antisense ODNs (once on day 1) with and without BDNF (twice; first infusion on day 1 and second infusion on day 2) on alcohol preference as measured by the two-bottle free-choice paradigm. Results represent the percentage of 7% v/v (ethanol in water solution) ethanol intake and percentage of water intake of total fluid intake (milliliters per day). Values are the mean ± SEM of six to eight rats in each group, and alcohol intake was measured for 4 d in the same group of rats.

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