Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors - PubMed (original) (raw)
. 2006 Aug 25;126(4):663-76.
doi: 10.1016/j.cell.2006.07.024. Epub 2006 Aug 10.
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- PMID: 16904174
- DOI: 10.1016/j.cell.2006.07.024
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Kazutoshi Takahashi et al. Cell. 2006.
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Abstract
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Comment in
- A transcriptional logic for nuclear reprogramming.
Rodolfa KT, Eggan K. Rodolfa KT, et al. Cell. 2006 Aug 25;126(4):652-5. doi: 10.1016/j.cell.2006.08.009. Cell. 2006. PMID: 16923385 - Achieving pluripotency.
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Papapetrou EP. Papapetrou EP. Science. 2016 Sep 2;353(6303):991-992. doi: 10.1126/science.aai7626. Science. 2016. PMID: 27701103 Free PMC article. - Stem cells to help the heart.
Sharma A. Sharma A. Science. 2020 Mar 13;367(6483):1206. doi: 10.1126/science.aba6111. Science. 2020. PMID: 32165579 No abstract available.
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