Nonblinking and long-lasting single-molecule fluorescence imaging - PubMed (original) (raw)
doi: 10.1038/nmeth934. Epub 2006 Oct 1.
Affiliations
- PMID: 17013382
- DOI: 10.1038/nmeth934
Nonblinking and long-lasting single-molecule fluorescence imaging
Ivan Rasnik et al. Nat Methods. 2006 Nov.
Abstract
Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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