The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing - PubMed (original) (raw)
. 2006 Dec;13(12):1078-83.
doi: 10.1038/nsmb1170. Epub 2006 Nov 12.
Affiliations
- PMID: 17099699
- DOI: 10.1038/nsmb1170
The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing
Janet G Yang et al. Nat Struct Mol Biol. 2006 Dec.
Abstract
Arrays of regularly spaced nucleosomes directly correlate with closed chromatin structures at silenced loci. The ATP-dependent chromatin-assembly factor (ACF) generates such arrays in vitro and is required for transcriptional silencing in vivo. A key unresolved question is how ACF 'measures' equal spacing between nucleosomes. We show that ACF senses flanking DNA length and transduces length information in an ATP-dependent manner to regulate the rate of nucleosome movement. Using fluorescence resonance energy transfer to follow nucleosome movement, we find that ACF can rapidly sample DNA on either side of a nucleosome and moves the longer flanking DNA across the nucleosome faster than the shorter flanking DNA. This generates a dynamic equilibrium in which nucleosomes having equal DNA on either side accumulate. Our results indicate that ACF generates the characteristic 50- to 60-base-pair internucleosomal spacing in silent chromatin by kinetically discriminating against shorter linker DNAs.
Comment in
- Lighting up nucleosome spacing.
Ferreira H, Owen-Hughes T. Ferreira H, et al. Nat Struct Mol Biol. 2006 Dec;13(12):1047-9. doi: 10.1038/nsmb1206-1047. Nat Struct Mol Biol. 2006. PMID: 17146458 No abstract available.
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