Role of hormone-sensitive lipase in beta-adrenergic remodeling of white adipose tissue - PubMed (original) (raw)
. 2007 Nov;293(5):E1188-97.
doi: 10.1152/ajpendo.00051.2007. Epub 2007 Aug 21.
Affiliations
- PMID: 17711991
- DOI: 10.1152/ajpendo.00051.2007
Free article
Role of hormone-sensitive lipase in beta-adrenergic remodeling of white adipose tissue
Emilio P Mottillo et al. Am J Physiol Endocrinol Metab. 2007 Nov.
Free article
Abstract
Free fatty acids (FFA) are important extracellular and intracellular signaling molecules and are thought to be involved in beta-adrenergic-induced remodeling of adipose tissue, which involves a transient inflammatory response followed by mitochondrial biogenesis and increased oxidative capacity. This work examined the role of hormone-sensitive lipase (HSL), a key enzyme of acylglycerol metabolism, in white adipose tissue (WAT) remodeling using genetic inactivation or pharmacological inhibition. Acute treatment with the beta(3)-adrenergic agonist CL-316,243 (CL) induced expression of inflammatory markers and caused extravasation of myeloid cells in WAT of wild-type (WT) mice. HSL-knockout (KO) mice had elevated inflammatory gene expression in the absence of stimulation, and acute injection of CL did not further recruit myeloid cells, nor did it further elevate inflammatory gene expression. Acute pharmacological inhibition of HSL with BAY 59-9435 (BAY) had no effect on inflammatory gene expression in WAT or in cultured 3T3-L1 adipocytes. However, BAY prevented induction of inflammatory cytokines by beta-adrenergic stimulation in WAT in vivo and in cultured 3T3-L1 adipocytes. Chronic CL treatment stimulated mitochondrial biogenesis, expanded oxidative capacity, and increased lipid droplet fragmentation in WT mice, and these effects were significantly impaired in HSL-KO mice. In contrast to HSL-KO mice, mice with defective signaling of Toll-like receptor 4, a putative FFA receptor, showed normal beta-adrenergic-induced remodeling of adipose tissue. Overall, results reveal the importance of HSL activity in WAT metabolic plasticity and inflammation.
Similar articles
- Hormone-sensitive lipase-independent adipocyte lipolysis during beta-adrenergic stimulation, fasting, and dietary fat loading.
Fortier M, Wang SP, Mauriège P, Semache M, Mfuma L, Li H, Levy E, Richard D, Mitchell GA. Fortier M, et al. Am J Physiol Endocrinol Metab. 2004 Aug;287(2):E282-8. doi: 10.1152/ajpendo.00203.2003. Am J Physiol Endocrinol Metab. 2004. PMID: 15271647 - Metabolic and cellular plasticity in white adipose tissue II: role of peroxisome proliferator-activated receptor-alpha.
Li P, Zhu Z, Lu Y, Granneman JG. Li P, et al. Am J Physiol Endocrinol Metab. 2005 Oct;289(4):E617-26. doi: 10.1152/ajpendo.00010.2005. Epub 2005 Jun 7. Am J Physiol Endocrinol Metab. 2005. PMID: 15941786 - beta(3)-Adrenoceptor agonists: potential, pitfalls and progress.
Arch JR. Arch JR. Eur J Pharmacol. 2002 Apr 12;440(2-3):99-107. doi: 10.1016/s0014-2999(02)01421-8. Eur J Pharmacol. 2002. PMID: 12007528 Review. - Hormone-sensitive lipase (HSL) expression and regulation in skeletal muscle.
Langfort J, Ploug T, Ihlemann J, Enevoldsen LH, Stallknecht B, Saldo M, Kjaer M, Holm C, Galbo H. Langfort J, et al. Adv Exp Med Biol. 1998;441:219-28. doi: 10.1007/978-1-4899-1928-1_20. Adv Exp Med Biol. 1998. PMID: 9781328 Review.
Cited by
- Macrophage function in adipose tissue homeostasis and metabolic inflammation.
Chavakis T, Alexaki VI, Ferrante AW Jr. Chavakis T, et al. Nat Immunol. 2023 May;24(5):757-766. doi: 10.1038/s41590-023-01479-0. Epub 2023 Apr 3. Nat Immunol. 2023. PMID: 37012544 Review. - An Overview of Hormone-Sensitive Lipase (HSL).
Althaher AR. Althaher AR. ScientificWorldJournal. 2022 Dec 8;2022:1964684. doi: 10.1155/2022/1964684. eCollection 2022. ScientificWorldJournal. 2022. PMID: 36530555 Free PMC article. Review. - Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulation.
Zhang X, Luo S, Wang M, Cao Q, Zhang Z, Huang Q, Li J, Deng Z, Liu T, Liu CL, Meppen M, Vromman A, Flavell RA, Hotamışlıgil GS, Liu J, Libby P, Liu Z, Shi GP. Zhang X, et al. Nat Commun. 2022 Dec 8;13(1):7582. doi: 10.1038/s41467-022-35256-8. Nat Commun. 2022. PMID: 36482059 Free PMC article. - Approaches to Measuring the Activity of Major Lipolytic and Lipogenic Enzymes In Vitro and Ex Vivo.
Wilhelm M, Rossmeislová L, Šiklová M. Wilhelm M, et al. Int J Mol Sci. 2022 Sep 21;23(19):11093. doi: 10.3390/ijms231911093. Int J Mol Sci. 2022. PMID: 36232405 Free PMC article. Review. - Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver.
Kim K, Kang JK, Jung YH, Lee SB, Rametta R, Dongiovanni P, Valenti L, Pajvani UB. Kim K, et al. Nat Commun. 2021 Mar 23;12(1):1822. doi: 10.1038/s41467-021-22106-2. Nat Commun. 2021. PMID: 33758172 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials