Characterization of the murine thrombospondin gene - PubMed (original) (raw)
Characterization of the murine thrombospondin gene
J Lawler et al. Genomics. 1991 Nov.
Abstract
Thrombospondin is an adhesive glycoprotein that supports cell attachment, spreading, and migration. The murine thrombospondin gene is approximately 18 kb in length and includes 22 exons. Interspecific backcross analysis using progeny derived from matings of (C57BL/6J x Mus spretus) F1 x C57BL/6J mice indicates that the thrombospondin gene is tightly linked to the Fshb, Actcl, Ltk, and B2M loci on murine chromosome 2. The sequence of the murine gene is very similar to that of the human gene in (1) regions of the promoter, (2) the coding region, and (3) the 3'-untranslated region. The predicted amino acid sequence of the mature murine thrombospondin subunit is 95.1% identical to that of the human. The sequences of these two species are most similar at the regions containing the type 1, 2, and 3 repeats as well as the COOH-terminal globular domain. The thrombospondin promoter is similar to the 5' flanking region of some housekeeping and growth control genes in that it contains multiple GC-rich regions and lacks a CAAT box. The presence of various consensus sequences suggests that thrombospondin gene expression is regulated by cAMP, cytokines, and steroid hormones.
Similar articles
- The thrombospondin-4 gene.
Newton G, Weremowicz S, Morton CC, Jenkins NA, Gilbert DJ, Copeland NG, Lawler J. Newton G, et al. Mamm Genome. 1999 Oct;10(10):1010-6. doi: 10.1007/s003359901149. Mamm Genome. 1999. PMID: 10501972 - Characterization of the mouse thrombospondin 2 gene.
Shingu T, Bornstein P. Shingu T, et al. Genomics. 1993 Apr;16(1):78-84. doi: 10.1006/geno.1993.1143. Genomics. 1993. PMID: 8486387 - Isolation and characterization of the mouse thrombospondin 3 (Thbs3) gene.
Bornstein P, Devarayalu S, Edelhoff S, Disteche CM. Bornstein P, et al. Genomics. 1993 Mar;15(3):607-13. doi: 10.1006/geno.1993.1114. Genomics. 1993. PMID: 8468055 - Cloning and sequencing of chicken thrombospondin.
Lawler J, Duquette M, Ferro P. Lawler J, et al. J Biol Chem. 1991 May 5;266(13):8039-43. J Biol Chem. 1991. PMID: 2022631 - Thrombospondins: structure and regulation of expression.
Bornstein P. Bornstein P. FASEB J. 1992 Nov;6(14):3290-9. doi: 10.1096/fasebj.6.14.1426766. FASEB J. 1992. PMID: 1426766 Review.
Cited by
- Human cytomegalovirus infection decreases expression of thrombospondin-1 independent of the tumor suppressor protein p53.
Cinatl J Jr, Kotchetkov R, Scholz M, Cinatl J, Vogel JU, Driever PH, Doerr HW. Cinatl J Jr, et al. Am J Pathol. 1999 Jul;155(1):285-92. doi: 10.1016/S0002-9440(10)65122-X. Am J Pathol. 1999. PMID: 10393860 Free PMC article. - Thrombospondin-1 is required for normal murine pulmonary homeostasis and its absence causes pneumonia.
Lawler J, Sunday M, Thibert V, Duquette M, George EL, Rayburn H, Hynes RO. Lawler J, et al. J Clin Invest. 1998 Mar 1;101(5):982-92. doi: 10.1172/JCI1684. J Clin Invest. 1998. PMID: 9486968 Free PMC article. - Thrombospondin 1 synthesis and function in wound repair.
DiPietro LA, Nissen NN, Gamelli RL, Koch AE, Pyle JM, Polverini PJ. DiPietro LA, et al. Am J Pathol. 1996 Jun;148(6):1851-60. Am J Pathol. 1996. PMID: 8669471 Free PMC article. - Receptor tyrosine kinase gene Tyro3 maps to mouse chromosome 2, closely linked to Ltk.
Liao X, Zhou R, Gilbert DJ, Copeland NG, Jenkins NA. Liao X, et al. Mamm Genome. 1996 May;7(5):395-6. doi: 10.1007/s003359900116. Mamm Genome. 1996. PMID: 8661736 No abstract available. - Thrombospondin-1, an inhibitor of angiogenesis, is regulated by progesterone in the human endometrium.
Iruela-Arispe ML, Porter P, Bornstein P, Sage EH. Iruela-Arispe ML, et al. J Clin Invest. 1996 Jan 15;97(2):403-12. doi: 10.1172/JCI118429. J Clin Invest. 1996. PMID: 8567961 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous