Insulin regulation of MCP-1 in human adipose tissue of obese and lean women - PubMed (original) (raw)
. 2008 May;294(5):E841-5.
doi: 10.1152/ajpendo.00653.2006. Epub 2008 Feb 12.
Affiliations
- PMID: 18270300
- DOI: 10.1152/ajpendo.00653.2006
Free article
Insulin regulation of MCP-1 in human adipose tissue of obese and lean women
Jukka Westerbacka et al. Am J Physiol Endocrinol Metab. 2008 May.
Free article
Abstract
CCL2 (MCP-1, monocyte chemoattractant protein 1) and CCL3 (MIP-1alpha, macrophage inflammatory protein 1alpha) are required for macrophage infiltration in adipose tissue. Insulin increases CCL2 expression in adipose tissue and in serum more in insulin-resistant obese than in insulin-sensitive lean mice, but whether this is true in humans is unknown. We compared basal expression and insulin regulation of CCL2 and CCL3 in adipose tissue and MCP-1 and MIP-1alpha in serum between insulin-resistant and insulin-sensitive human subjects. Subcutaneous adipose tissue biopsies and blood samples were obtained before and at the end of 6 h of in vivo euglycemic hyperinsulinemia (maintained by the insulin clamp technique) in 11 lean insulin-sensitive and 10 obese insulin-resistant women, and before and after a 6-h saline infusion in 8 women. Adipose tissue mRNA concentrations of monocyte/macrophage markers CD68, EMR1, ITGAM, ADAM8, chemokines CCL2 and CCL3, and housekeeping gene ribosomal protein large P0 (RPLP0) were measured by means of real-time PCR at baseline. In addition, mRNA concentrations of CCL2, CCL3, and RPLP0 were measured after insulin infusion. Levels of MCP-1 and MIP-1alpha were determined in serum, and protein concentration of MCP-1 was determined in adipose tissue at baseline and after insulin infusion. Basally, expression of the macrophage markers CD68 and EMR1 were increased in adipose tissue of insulin-resistant subjects. Insulin increased MCP-1 gene and protein expression significantly more in the insulin-resistant than in the insulin-sensitive subjects. Basally expression of CCL2 and CCL3 and expression of macrophage markers CD68 and ITGAM were significantly correlated. In serum, MCP-1 decreased significantly in insulin-sensitive but not insulin-resistant subjects. MIP-1alpha was undetectable in serum. Insulin regulation of CCL2 differs between insulin-sensitive and -resistant subjects in a direction that could exacerbate adipose tissue inflammation.
Similar articles
- Increased expression of the macrophage markers and of 11beta-HSD-1 in subcutaneous adipose tissue, but not in cultured monocyte-derived macrophages, is associated with liver fat in human obesity.
Makkonen J, Westerbacka J, Kolak M, Sutinen J, Cornér A, Hamsten A, Fisher RM, Yki-Järvinen H. Makkonen J, et al. Int J Obes (Lond). 2007 Oct;31(10):1617-25. doi: 10.1038/sj.ijo.0803635. Epub 2007 Apr 24. Int J Obes (Lond). 2007. PMID: 17452990 - Diet induction of monocyte chemoattractant protein-1 and its impact on obesity.
Chen A, Mumick S, Zhang C, Lamb J, Dai H, Weingarth D, Mudgett J, Chen H, MacNeil DJ, Reitman ML, Qian S. Chen A, et al. Obes Res. 2005 Aug;13(8):1311-20. doi: 10.1038/oby.2005.159. Obes Res. 2005. PMID: 16129712 - CCR3, CCR2A and macrophage inflammatory protein (MIP)-1a, monocyte chemotactic protein-1 (MCP-1) in the mouse hippocampus during and after pilocarpine-induced status epilepticus (PISE).
Xu JH, Long L, Tang YC, Zhang JT, Hut HT, Tang FR. Xu JH, et al. Neuropathol Appl Neurobiol. 2009 Oct;35(5):496-514. doi: 10.1111/j.1365-2990.2009.01022.x. Neuropathol Appl Neurobiol. 2009. PMID: 19490431 - [Macrophages, inflammation, adipose tissue, obesity and insulin resistance].
Bastarrachea RA, López-Alvarenga JC, Bolado-García VE, Téllez-Mendoza J, Laviada-Molina H, Comuzzie AG. Bastarrachea RA, et al. Gac Med Mex. 2007 Nov-Dec;143(6):505-12. Gac Med Mex. 2007. PMID: 18269082 Review. Spanish. - Monocyte chemotactic protein-1 and its role in insulin resistance.
Sell H, Eckel J. Sell H, et al. Curr Opin Lipidol. 2007 Jun;18(3):258-62. doi: 10.1097/MOL.0b013e3281338546. Curr Opin Lipidol. 2007. PMID: 17495598 Review.
Cited by
- Identification of hub genes associated with Helicobacter pylori infection and type 2 diabetes mellitus: A pilot bioinformatics study.
Chen H, Zhang GX, Zhou XY. Chen H, et al. World J Diabetes. 2024 Feb 15;15(2):170-185. doi: 10.4239/wjd.v15.i2.170. World J Diabetes. 2024. PMID: 38464370 Free PMC article. - Ketosis Suppression and Ageing (KetoSAge): The Effects of Suppressing Ketosis in Long Term Keto-Adapted Non-Athletic Females.
Cooper ID, Kyriakidou Y, Edwards K, Petagine L, Seyfried TN, Duraj T, Soto-Mota A, Scarborough A, Jacome SL, Brookler K, Borgognoni V, Novaes V, Al-Faour R, Elliott BT. Cooper ID, et al. Int J Mol Sci. 2023 Oct 26;24(21):15621. doi: 10.3390/ijms242115621. Int J Mol Sci. 2023. PMID: 37958602 Free PMC article. - Adipokines, Hepatokines and Myokines: Focus on Their Role and Molecular Mechanisms in Adipose Tissue Inflammation.
Ren Y, Zhao H, Yin C, Lan X, Wu L, Du X, Griffiths HR, Gao D. Ren Y, et al. Front Endocrinol (Lausanne). 2022 Jul 14;13:873699. doi: 10.3389/fendo.2022.873699. eCollection 2022. Front Endocrinol (Lausanne). 2022. PMID: 35909571 Free PMC article. Review. - Physiological Changes and Pathological Pain Associated with Sedentary Lifestyle-Induced Body Systems Fat Accumulation and Their Modulation by Physical Exercise.
Verdú E, Homs J, Boadas-Vaello P. Verdú E, et al. Int J Environ Res Public Health. 2021 Dec 17;18(24):13333. doi: 10.3390/ijerph182413333. Int J Environ Res Public Health. 2021. PMID: 34948944 Free PMC article. Review. - Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo Middle-Aged Women?
Balcázar-Hernandez L, Basurto L, Manuel-Apolinar L, Vega-García S, Basurto-Acevedo N, Martínez-Murillo C, Sánchez-Arenas R. Balcázar-Hernandez L, et al. Metabolites. 2021 Nov 11;11(11):771. doi: 10.3390/metabo11110771. Metabolites. 2021. PMID: 34822430 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous