Clinical role of p16INK4a expression in liquid-based cervical cytology: correlation with HPV testing and histologic diagnosis - PubMed (original) (raw)
Clinical role of p16INK4a expression in liquid-based cervical cytology: correlation with HPV testing and histologic diagnosis
Maria Benevolo et al. Am J Clin Pathol. 2008 Apr.
Abstract
p16INK4a is overexpressed in high-risk human papillomavirus (HR-HPV)-infected preneoplastic and neoplastic lesions of the uterine cervix. Our aim was to verify whether p16 is a diagnostic marker also in cervical liquid-based cytology. We performed p16 immunocytochemical analysis and the Hybrid Capture 2 (HC2) test (Digene, Gaithersburg, MD) for HR-HPV infection in 471 ThinPrep-processed (Cytyc, Boxborough, MA) cervicovaginal samples and correlated the results with histologic findings. A total of 32.3% of the specimens showed p16 immunoreactivity, whereas the HC2 test was positive in 41.2% of the cases (65.2% concordance rate). Correlating the cytologic, p16, and HPV results with histologic findings revealed HC2 as the most sensitive test for a diagnosis of cervical intraepithelial neoplasia 2 or worse, whereas cytologic examination was the most specific. The positive predictive value was significantly higher for cytologic examination than for p16 and HR-HPV testing. These data suggest that p16 evaluation in ThinPrep samples does not have better clinical effectiveness for identifying high-grade lesions than conventional morphologic examination and HPV testing.
Similar articles
- p16INK4a immunocytochemistry versus human papillomavirus testing for triage of women with minor cytologic abnormalities: a systematic review and meta-analysis.
Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M. Roelens J, et al. Cancer Cytopathol. 2012 Oct 25;120(5):294-307. doi: 10.1002/cncy.21205. Epub 2012 Jun 14. Cancer Cytopathol. 2012. PMID: 22700382 Free PMC article. Review. - Liquid-based cytology--new possibilities in the diagnosis of cervical lesions.
Juric D, Mahovlić V, Rajhvajn S, Ovanin-Rakić A, Skopljanac-Macina L, Barisić A, Projić IS, Babić D, Susa M, Corusić A, Oresković S. Juric D, et al. Coll Antropol. 2010 Mar;34(1):19-24. Coll Antropol. 2010. PMID: 20432728 - The predictive value of p16(INK4a) and hybrid capture 2 human papillomavirus testing for high-grade cervical intraepithelial neoplasia.
Guo M, Hu L, Baliga M, He Z, Hughson MD. Guo M, et al. Am J Clin Pathol. 2004 Dec;122(6):894-901. doi: 10.1309/0DGG-QBDQ-AMJC-JBXB. Am J Clin Pathol. 2004. PMID: 15539381 - Diagnostic utility of p16INK4a: a reappraisal of its use in cervical biopsies.
Mulvany NJ, Allen DG, Wilson SM. Mulvany NJ, et al. Pathology. 2008 Jun;40(4):335-44. doi: 10.1080/00313020802035907. Pathology. 2008. PMID: 18446622 Review.
Cited by
- Multicentre Evaluation of Hepika Test Clinical Accuracy in Diagnosing HPV-Induced Cancer and Precancerous Lesions of the Uterine Cervix.
Gustinucci D, Ciccocioppo L, Coppola L, Negri G, Zannoni G, Passamonti B, Cesarini E, Ianzano C, Andreano T, Pireddu A, Giorgi-Rossi P. Gustinucci D, et al. Diagnostics (Basel). 2021 Mar 30;11(4):619. doi: 10.3390/diagnostics11040619. Diagnostics (Basel). 2021. PMID: 33808260 Free PMC article. - Cervical cancer screening in developing countries at a crossroad: Emerging technologies and policy choices.
Catarino R, Petignat P, Dongui G, Vassilakos P. Catarino R, et al. World J Clin Oncol. 2015 Dec 10;6(6):281-90. doi: 10.5306/wjco.v6.i6.281. World J Clin Oncol. 2015. PMID: 26677441 Free PMC article. Review. - Combined detection of p16(INK4a) and IMP3 increase the concordance rate between cervical cytologic and histologic diagnosis.
Wei Q, Fu B, Liu J, Xu J, Zhao T. Wei Q, et al. Int J Clin Exp Pathol. 2013 Jul 15;6(8):1549-57. Print 2013. Int J Clin Exp Pathol. 2013. PMID: 23923073 Free PMC article. - Relative accuracy of cervical and anal cytology for detection of high grade lesions by colposcope guided biopsy: a cut-point meta-analytic comparison.
Cachay ER, Agmas W, Mathews WC. Cachay ER, et al. PLoS One. 2012;7(7):e38956. doi: 10.1371/journal.pone.0038956. Epub 2012 Jul 25. PLoS One. 2012. PMID: 22848345 Free PMC article. Review. - p16INK4a immunocytochemistry versus human papillomavirus testing for triage of women with minor cytologic abnormalities: a systematic review and meta-analysis.
Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M. Roelens J, et al. Cancer Cytopathol. 2012 Oct 25;120(5):294-307. doi: 10.1002/cncy.21205. Epub 2012 Jun 14. Cancer Cytopathol. 2012. PMID: 22700382 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials