Expression analysis of G Protein-Coupled Receptors in mouse macrophages - PubMed (original) (raw)

Expression analysis of G Protein-Coupled Receptors in mouse macrophages

Jane E Lattin et al. Immunome Res. 2008.

Abstract

Background: Monocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity. In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages to identify family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by an inflammatory stimulus, the bacterial product lipopolysaccharide (LPS).

Results: Several members of the P2RY family had striking expression patterns in macrophages; P2ry6 mRNA was essentially expressed in a macrophage-specific fashion, whilst P2ry1 and P2ry5 mRNA levels were strongly down-regulated by LPS. Expression of several other GPCRs was either restricted to macrophages (e.g. Gpr84) or to both macrophages and neural tissues (e.g. P2ry12, Gpr85). The GPCR repertoire expressed by bone marrow-derived macrophages and thioglycollate-elicited peritoneal macrophages had some commonality, but there were also several GPCRs preferentially expressed by either cell population.

Conclusion: The constitutive or regulated expression in macrophages of several GPCRs identified in this study has not previously been described. Future studies on such GPCRs and their agonists are likely to provide important insights into macrophage biology, as well as novel inflammatory pathways that could be future targets for drug discovery.

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Figures

Figure 1

Figure 1

Macrophage-restricted GPCRs. Micro-array analysis of Gpr85 (A), P2ry6 (B) and Gpr84 (C) mRNA expression across a panel of 91 murine cell types and tissues. Data points show normalised values and similar cell types are grouped according to bar colour; blue indicates primary macrophage cell types, purple indicates bone-related cell types, red indicates other immune cell types, green indicates stem cell populations, orange indicates whole tissue samples, yellow indicates neuronal and retinal cell types and pink indicates cell lines. Additional file 3 gives details of the 91 cell types and tissues profiled.

Figure 2

Figure 2

GPCRs differentially regulated in BMM and TEPM. Micro-array analysis of Calcrl (A), Gprc5b (B) and P2ry14 (C) mRNA expression across a panel of 91 murine cell types and tissues. Data points show normalised values and similar cell types are grouped according to bar colour; blue indicates primary macrophage cell types, purple indicates bone-related cell types, red indicates other immune cell types, green indicates stem cell populations, orange indicates whole tissue samples, yellow indicates neuronal and retinal cell types and pink indicates cell lines. Additional file 3 gives details of the 91 cell types and tissues profiled.

Figure 3

Figure 3

GPCRs regulated by LPS in macrophages. Micro-array expression analysis of Gpr84 (A), P2ry1 (B) and Prokr1 (C) mRNA in BMM and TEPM over a timecourse of 0, 2, 6, and 24 h, and 0, 1 and 7 h, respectively. Data points show gene expression relative to untreated control for each cell population (0 h).

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