Rel-associated pp40: an inhibitor of the rel family of transcription factors - PubMed (original) (raw)

Comparative Study

. 1991 Sep 13;253(5025):1268-71.

doi: 10.1126/science.1891714.

Affiliations

• PMID: 1891714
• DOI: 10.1126/science.1891714

Comparative Study

Rel-associated pp40: an inhibitor of the rel family of transcription factors

N Davis et al. Science. 1991.

Abstract

The Rel-associated protein pp40 is functionally related to I kappa B, an inhibitor of the transcription factor NF-kappa B. Purified pp40 inhibits the DNA binding activity of the NF-kappa B protein complex (p50:p65 heterodimers), p50:c-Rel heteromers, and c-Rel homodimers. The sequence of the complementary DNA encoding pp40 revealed similarity to the gene encoding MAD-3, a protein with mammalian I kappa B-like activity. Protein sequencing of I kappa B purified from rabbit lung confirmed that MAD-3 encodes a protein similar to I kappa B. The sequence similarity between MAD-3 and pp40 includes a casein kinase II and consensus tyrosine phosphorylation site, as well as five repeats of a sequence found in the human erythrocyte protein ankyrin. These results suggest that rel-related transcription factors, which are capable of cytosolic to nuclear translocation, may be held in the cytosol by interaction with related cytoplasmic anchor molecules.

PubMed Disclaimer

Similar articles

• Direct association of pp40/I kappa B beta with rel/NF-kappa B transcription factors: role of ankyrin repeats in the inhibition of DNA binding activity.
  Inoue J, Kerr LD, Rashid D, Davis N, Bose HR Jr, Verma IM. Inoue J, et al. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4333-7. doi: 10.1073/pnas.89.10.4333. Proc Natl Acad Sci U S A. 1992. PMID: 1533932 Free PMC article.
• The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation.
  Kerr LD, Inoue J, Davis N, Link E, Baeuerle PA, Bose HR Jr, Verma IM. Kerr LD, et al. Genes Dev. 1991 Aug;5(8):1464-76. doi: 10.1101/gad.5.8.1464. Genes Dev. 1991. PMID: 1907941
• I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding.
  Ganchi PA, Sun SC, Greene WC, Ballard DW. Ganchi PA, et al. Mol Biol Cell. 1992 Dec;3(12):1339-52. doi: 10.1091/mbc.3.12.1339. Mol Biol Cell. 1992. PMID: 1493333 Free PMC article.
• Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.
  Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S. Verma IM, et al. Genes Dev. 1995 Nov 15;9(22):2723-35. doi: 10.1101/gad.9.22.2723. Genes Dev. 1995. PMID: 7590248 Review. No abstract available.
• Rel/NF-kappa B/I kappa B story.
  Miyamoto S, Verma IM. Miyamoto S, et al. Adv Cancer Res. 1995;66:255-92. Adv Cancer Res. 1995. PMID: 7793317 Review. No abstract available.

Cited by

• Phosphorylation of I kappa B alpha precedes but is not sufficient for its dissociation from NF-kappa B.
  DiDonato JA, Mercurio F, Karin M. DiDonato JA, et al. Mol Cell Biol. 1995 Mar;15(3):1302-11. doi: 10.1128/MCB.15.3.1302. Mol Cell Biol. 1995. PMID: 7862124 Free PMC article.
• Regulation of IkappaB beta in WEHI 231 mature B cells.
  Phillips RJ, Ghosh S. Phillips RJ, et al. Mol Cell Biol. 1997 Aug;17(8):4390-6. doi: 10.1128/MCB.17.8.4390. Mol Cell Biol. 1997. PMID: 9234697 Free PMC article.
• The relocalization of v-Rel from the nucleus to the cytoplasm coincides with induction of expression of Ikba and nfkb1 and stabilization of I kappa B-alpha.
  Hrdlicková R, Nehyba J, Roy A, Humphries EH, Bose HR Jr. Hrdlicková R, et al. J Virol. 1995 Jan;69(1):403-13. doi: 10.1128/JVI.69.1.403-413.1995. J Virol. 1995. PMID: 7983736 Free PMC article.
• Differential pp40I kappa B-beta inhibition of DNA binding by rel proteins.
  Diehl JA, McKinsey TA, Hannink M. Diehl JA, et al. Mol Cell Biol. 1993 Mar;13(3):1769-78. doi: 10.1128/mcb.13.3.1769-1778.1993. Mol Cell Biol. 1993. PMID: 8441412 Free PMC article.
• Identification of a NFKBIA polymorphism associated with lower NFKBIA protein levels and poor survival outcomes in patients with glioblastoma multiforme.
  Zhao Z, Zhong X, Wu T, Yang T, Chen G, Xie X, Wei Y, Ye M, Zhou Y, Du Z. Zhao Z, et al. Int J Mol Med. 2014 Nov;34(5):1233-40. doi: 10.3892/ijmm.2014.1932. Epub 2014 Sep 12. Int J Mol Med. 2014. PMID: 25215581 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program