Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli - PubMed (original) (raw)
. 2009 Apr;296(4):G868-75.
doi: 10.1152/ajpgi.90511.2008. Epub 2009 Jan 29.
Affiliations
- PMID: 19179624
- DOI: 10.1152/ajpgi.90511.2008
Free article
Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli
Xuelian Ma et al. Am J Physiol Gastrointest Liver Physiol. 2009 Apr.
Free article
Erratum in
- Am J Physiol Gastrointest Liver Physiol. 2011 Jul;301(1):G195
Abstract
Lactobacillus species ingestion can decrease autonomic responses and spinal fiber discharge to nociceptive colorectal distension (CRD), even in the absence of inflammation. The present study aimed to determine whether dorsal root ganglion (DRG) somas could be a locus where the antinociceptive probiotic may have an effect. Healthy rats were fed with Lactobacillus reuteri or vehicle control for 9 days whereupon they were anesthetized, and intermittent distal colonic CRD at 80 mmHg distension was either performed for 1 h or not. The animals were immediately euthanized and patch-clamp recordings taken after isolation and overnight culture from those DRG that projected to the distal colon. CRD decreased the threshold for action potential generation and increased the number of spikes discharged during a standard depolarizing test stimulus, and this effect was blocked by prior probiotic ingestion. The increase in excitability was paralleled by an increase in DRG capacitance, which was not altered by Lactobacillus reuteri ingestion. CRD did not increase tissue weight or myeloperoxidase activity. We suggest that the effects of CRD may have been caused by activity-dependent neurotransmission between DRG somas. CRD evoked increases in action potential upstroke speed, which suggests that it may also have led to augmentation of sodium channel conductances. Probiotic ingestion may have interfered with this hypothetical mechanism since it blocked the effect of CRD on the action potential.
Similar articles
- The TRPV1 channel in rodents is a major target for antinociceptive effect of the probiotic Lactobacillus reuteri DSM 17938.
Perez-Burgos A, Wang L, McVey Neufeld KA, Mao YK, Ahmadzai M, Janssen LJ, Stanisz AM, Bienenstock J, Kunze WA. Perez-Burgos A, et al. J Physiol. 2015 Sep 1;593(17):3943-57. doi: 10.1113/JP270229. Epub 2015 Jul 20. J Physiol. 2015. PMID: 26084409 Free PMC article. - Inhibitory effects of Lactobacillus reuteri on visceral pain induced by colorectal distension in Sprague-Dawley rats.
Kamiya T, Wang L, Forsythe P, Goettsche G, Mao Y, Wang Y, Tougas G, Bienenstock J. Kamiya T, et al. Gut. 2006 Feb;55(2):191-6. doi: 10.1136/gut.2005.070987. Epub 2005 Dec 16. Gut. 2006. PMID: 16361309 Free PMC article. - Lactobacillus reuteri enhances excitability of colonic AH neurons by inhibiting calcium-dependent potassium channel opening.
Kunze WA, Mao YK, Wang B, Huizinga JD, Ma X, Forsythe P, Bienenstock J. Kunze WA, et al. J Cell Mol Med. 2009 Aug;13(8B):2261-2270. doi: 10.1111/j.1582-4934.2009.00686.x. Epub 2009 Feb 4. J Cell Mol Med. 2009. PMID: 19210574 Free PMC article. - Changes of the neuropeptides content and gene expression in spinal cord and dorsal root ganglion after noxious colorectal distension.
Lu CL, Pasricha PJ, Hsieh JC, Lu RH, Lai CR, Wu LL, Chang FY, Lee SD. Lu CL, et al. Regul Pept. 2005 Nov;131(1-3):66-73. doi: 10.1016/j.regpep.2005.06.008. Regul Pept. 2005. PMID: 16084604 - Lactobacillus reuteri ingestion and IK(Ca) channel blockade have similar effects on rat colon motility and myenteric neurones.
Wang B, Mao YK, Diorio C, Wang L, Huizinga JD, Bienenstock J, Kunze W. Wang B, et al. Neurogastroenterol Motil. 2010 Jan;22(1):98-107, e33. doi: 10.1111/j.1365-2982.2009.01384.x. Epub 2009 Sep 29. Neurogastroenterol Motil. 2010. PMID: 19788711
Cited by
- Global research progress of visceral hypersensitivity and irritable bowel syndrome: bibliometrics and visualized analysis.
Tian S, Zhang H, Chen S, Wu P, Chen M. Tian S, et al. Front Pharmacol. 2023 May 2;14:1175057. doi: 10.3389/fphar.2023.1175057. eCollection 2023. Front Pharmacol. 2023. PMID: 37201020 Free PMC article. - When the microbiome helps the brain-current evidence.
Drljača J, Milošević N, Milanović M, Abenavoli L, Milić N. Drljača J, et al. CNS Neurosci Ther. 2023 Jun;29 Suppl 1(Suppl 1):43-58. doi: 10.1111/cns.14076. Epub 2023 Jan 4. CNS Neurosci Ther. 2023. PMID: 36601680 Free PMC article. Review. - Glioma and the gut-brain axis: opportunities and future perspectives.
Dono A, Nickles J, Rodriguez-Armendariz AG, McFarland BC, Ajami NJ, Ballester LY, Wargo JA, Esquenazi Y. Dono A, et al. Neurooncol Adv. 2022 Apr 14;4(1):vdac054. doi: 10.1093/noajnl/vdac054. eCollection 2022 Jan-Dec. Neurooncol Adv. 2022. PMID: 35591978 Free PMC article. Review. - One Giant Leap from Mouse to Man: The Microbiota-Gut-Brain Axis in Mood Disorders and Translational Challenges Moving towards Human Clinical Trials.
Forssten SD, Ouwehand AC, Griffin SM, Patterson E. Forssten SD, et al. Nutrients. 2022 Jan 27;14(3):568. doi: 10.3390/nu14030568. Nutrients. 2022. PMID: 35276927 Free PMC article. Review. - Probiotics, Prebiotics, and Synbiotics: Implications and Beneficial Effects against Irritable Bowel Syndrome.
Simon E, Călinoiu LF, Mitrea L, Vodnar DC. Simon E, et al. Nutrients. 2021 Jun 20;13(6):2112. doi: 10.3390/nu13062112. Nutrients. 2021. PMID: 34203002 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials