Analyzing serum-stimulated prostate cancer cell lines after low-fat, high-fiber diet and exercise intervention - PubMed (original) (raw)

Analyzing serum-stimulated prostate cancer cell lines after low-fat, high-fiber diet and exercise intervention

Sherry Soliman et al. Evid Based Complement Alternat Med. 2011.

Abstract

Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for these anticancer effects. Again, the intervention slowed growth and increased apoptosis in LNCaP cells; responses that were eliminated when IGF-I was added back to the post-intervention samples. The p53 protein content was increased and NFκB activation reduced in the post serum-stimulated LNCaP cells. Similar results were observed when the IGF-I receptor was blocked in the pre-intervention serum. In androgen-independent PC-3 cells, growth was reduced while none of the other factors were changed by the intervention. We conclude that diet and exercise intervention might help prevent clinical PCa as well as aid in the treatment of PCa during the early stages of development.

PubMed Disclaimer

Figures

Figure 1

Figure 1

Effect of diet and exercise intervention, as well as various blockers, on the growth of serum-stimulated, androgen-dependent LNCaP cells.

Figure 2

Figure 2

Effect of diet and exercise intervention, as well as various blockers, on apoptosis of serum-stimulated, androgen-dependent LNCaP cells.

Figure 3

Figure 3

Effect of diet and exercise intervention, as well as various blockers, on the growth of serum-stimulated, androgen-independent PC-3 cells.

Figure 4

Figure 4

Effect of diet and exercise intervention, as well as various blockers, on apoptosis of serum-stimulated, androgen-independent PC-3 cells.

Figure 5

Figure 5

Effect of adding back IGF-I to the post diet and exercise serum on growth and apoptosis of LNCaP cells.

Figure 6

Figure 6

A hypothetical model to explain how adopting a low-fat, high-fiber diet and daily exercise might reduce the risk for PCa by reducing prostate inflammation and IGF-I/insulin signaling, IGF-I, insulin-like growth factor-I; PI3 kinase, phosphatidylinositol-3 kinase; Akt, protein kinase B; MAPK, mitogen-activated protein kinase (p38); COX-2, cyclooxygenase-2; ROS, reactive oxygen species; IL-1, interlukin-1, TNF_α_, tumor necrosis factor-alpha.

References

    1. Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2006. CA: A Cancer Journal for Clinicians. 2006;56(2):106–130. - PubMed
    1. Hsing AW, Tsao L, Devesa SS. International trends and patterns of prostate cancer incidence and mortality. International Journal of Cancer. 2000;85(1):60–67. - PubMed
    1. Muir CS, Nectoux J, Staszewski J. The epidemiology of prostatic cancer. Geographical distribution and time-trends. Acta Oncologica. 1991;30(2):133–140. - PubMed
    1. Shimizu H, Ross RK, Bernstein L, Yatani R, Henderson BE, Mack TM. Cancers of the prostate and breast among Japanese and white immigrants in Los Angeles County. British Journal of Cancer. 1991;63(6):963–966. - PMC - PubMed
    1. Moyad MA. Dietary fat reduction to reduce prostate cancer risk: controlled enthusiasm, learning a lesson from breast or other cancers, and the big picture. Urology. 2002;59(4, supplement 1):51–62. - PubMed

LinkOut - more resources