Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential - PubMed (original) (raw)
. 2009 Jun 18;113(25):6342-50.
doi: 10.1182/blood-2008-12-192054. Epub 2009 Apr 17.
Michael R Copley, Claudia Benz, Stefan Wöhrer, Brad J Dykstra, Elaine Ma, John Cheyne, Yongjun Zhao, Michelle B Bowie, Yun Zhao, Maura Gasparetto, Allen Delaney, Clayton Smith, Marco Marra, Connie J Eaves
Affiliations
- PMID: 19377048
- DOI: 10.1182/blood-2008-12-192054
Free article
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
David G Kent et al. Blood. 2009.
Free article
Abstract
Hematopoietic stem cells (HSCs) are generally defined by their dual properties of pluripotency and extensive self-renewal capacity. However, a lack of experimental clarity as to what constitutes extensive self-renewal capacity coupled with an absence of methods to prospectively isolate long-term repopulating cells with defined self-renewal activities has made it difficult to identify the essential components of the self-renewal machinery and investigate their regulation. We now show that cells capable of repopulating irradiated congenic hosts for 4 months and producing clones of cells that can be serially transplanted are selectively and highly enriched in the CD150(+) subset of the EPCR(+)CD48(-)CD45(+) fraction of mouse fetal liver and adult bone marrow cells. In contrast, cells that repopulate primary hosts for the same period but show more limited self-renewal activity are enriched in the CD150(-) subset. Comparative transcriptome analyses of these 2 subsets with each other and with HSCs whose self-renewal activity has been rapidly extinguished in vitro revealed 3 new genes (VWF, Rhob, Pld3) whose elevated expression is a consistent and selective feature of the long-term repopulating cells with durable self-renewal capacity. These findings establish the identity of a phenotypically and molecularly distinct class of pluripotent hematopoietic cells with lifelong self-renewal capacity.
Similar articles
- Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150.
Papathanasiou P, Attema JL, Karsunky H, Xu J, Smale ST, Weissman IL. Papathanasiou P, et al. Stem Cells. 2009 Oct;27(10):2498-508. doi: 10.1002/stem.170. Stem Cells. 2009. PMID: 19593793 Free PMC article. - Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors.
Kim I, He S, Yilmaz OH, Kiel MJ, Morrison SJ. Kim I, et al. Blood. 2006 Jul 15;108(2):737-44. doi: 10.1182/blood-2005-10-4135. Epub 2006 Mar 28. Blood. 2006. PMID: 16569764 Free PMC article. - SLAM family markers resolve functionally distinct subpopulations of hematopoietic stem cells and multipotent progenitors.
Oguro H, Ding L, Morrison SJ. Oguro H, et al. Cell Stem Cell. 2013 Jul 3;13(1):102-16. doi: 10.1016/j.stem.2013.05.014. Cell Stem Cell. 2013. PMID: 23827712 Free PMC article. - Quantitative assessment of the stem cell self-renewal capacity.
Nakauchi H, Sudo K, Ema H. Nakauchi H, et al. Ann N Y Acad Sci. 2001 Jun;938:18-24; discussion 24-5. doi: 10.1111/j.1749-6632.2001.tb03570.x. Ann N Y Acad Sci. 2001. PMID: 11458506 Review. - Hematopoietic stem cells.
Hawley RG, Ramezani A, Hawley TS. Hawley RG, et al. Methods Enzymol. 2006;419:149-79. doi: 10.1016/S0076-6879(06)19007-2. Methods Enzymol. 2006. PMID: 17141055 Free PMC article. Review.
Cited by
- Sheng Xue Ning as a Novel Agent that Promotes SCF-Driven Hematopoietic Stem/Progenitor Cell Proliferation to Promote Erythropoiesis.
Zeng Y, Li C, Yang F, Zhang L, Xu W, Wang L, Wu A, Zou W, Wu J, Huang F. Zeng Y, et al. Biomolecules. 2024 Sep 11;14(9):1147. doi: 10.3390/biom14091147. Biomolecules. 2024. PMID: 39334913 Free PMC article. - Clonal analysis of fetal hematopoietic stem/progenitor cells reveals how post-transplantation capabilities are distributed.
Stonehouse OJ, Biben C, Weber TS, Garnham A, Fennell KA, Farley A, Terreaux AF, Alexander WS, Dawson MA, Naik SH, Taoudi S. Stonehouse OJ, et al. Stem Cell Reports. 2024 Aug 13;19(8):1189-1204. doi: 10.1016/j.stemcr.2024.07.003. Epub 2024 Aug 1. Stem Cell Reports. 2024. PMID: 39094562 Free PMC article. - Neurobeachin regulates hematopoietic progenitor differentiation and survival by modulating Notch activity.
Ganuza M, Morales-Hernández A, Van Huizen A, Chabot A, Hall T, Caprio C, Finkelstein D, Kilimann MW, McKinney-Freeman S. Ganuza M, et al. Blood Adv. 2024 Aug 13;8(15):4129-4143. doi: 10.1182/bloodadvances.2023012426. Blood Adv. 2024. PMID: 38905595 Free PMC article. - Early megakaryocyte lineage-committed progenitors in adult mouse bone marrow.
Liu Z, Wang J, Ma Y, Xie M, Wu P, Zhang S, Wang X, Dong F, Cheng H, Zhu P, Han M, Ema H. Liu Z, et al. Blood Sci. 2024 May 7;6(2):e00187. doi: 10.1097/BS9.0000000000000187. eCollection 2024 Apr. Blood Sci. 2024. PMID: 38721470 Free PMC article. - Endothelial Protein C Receptor and Its Impact on Rheumatic Disease.
O'Hehir ZD, Lynch T, O'Neill S, March L, Xue M. O'Hehir ZD, et al. J Clin Med. 2024 Mar 31;13(7):2030. doi: 10.3390/jcm13072030. J Clin Med. 2024. PMID: 38610795 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials
Miscellaneous