Molecular characterization of human retinal progenitor cells - PubMed (original) (raw)
. 2009 Dec;50(12):5901-8.
doi: 10.1167/iovs.08-3067. Epub 2009 Jun 24.
Affiliations
- PMID: 19553622
- DOI: 10.1167/iovs.08-3067
Molecular characterization of human retinal progenitor cells
Scott Schmitt et al. Invest Ophthalmol Vis Sci. 2009 Dec.
Abstract
Purpose: To examine the molecular profile of fetal human retinal progenitor cells (hRPCs) expanded in vitro and those grown in a co-culture system with mouse retina through the analysis of protein and gene expression and neurotransmitter-stimulated calcium dynamics.
Methods: hRPCS were isolated from human retina of 14 to 18 weeks gestational age (GA) and expanded in vitro. Immunoblot, microarray, and immunocytochemistry (ICC) assays were performed on undifferentiated hRPCs and those co-cultured with mouse retinas for 2 weeks. Cell function was assessed by using calcium imaging.
Results: The ICC results showed a gradual decrease in the percentages of KI67-, SOX2-, and vimentin-positive cells from passages (P) 1 to P6, whereas a sustained expression of nestin and PAX6 was observed through P6. Microarray analysis of P1 hRPCs showed the expression of early retinal developmental genes: VIM (vimentin), KI67, NES (nestin), PAX6, SOX2, HES5, GNL3, OTX2, DACH1, SIX6, and CHX10 (VSX2). At P6, hRPCs continued to express VIM, KI67, NES, PAX6, SOX2, GNL3, and SIX6. On co-culture, there was a significant increase in the expression of MKI67, PAX6, SOX2, GNL3, SIX3, and RHO (rhodopsin). Calcium imaging showed a functional response to excitatory neurotransmitters.
Conclusions: Fetal-derived hRPCs show molecular characteristics indicative of a retinal progenitor state up to P6 (latest passage studied). They show a progressive decrease in the expression of immature markers as they reach P6. These cells are functional, respond to excitatory neurotransmitters, and exhibit changes in expression patterns in response to co-culture with mouse retina.
Similar articles
- Growth kinetics and transplantation of human retinal progenitor cells.
Aftab U, Jiang C, Tucker B, Kim JY, Klassen H, Miljan E, Sinden J, Young M. Aftab U, et al. Exp Eye Res. 2009 Sep;89(3):301-10. doi: 10.1016/j.exer.2009.03.025. Epub 2009 Jun 12. Exp Eye Res. 2009. PMID: 19524569 - Isolation of retinal progenitor cells from post-mortem human tissue and comparison with autologous brain progenitors.
Klassen H, Ziaeian B, Kirov II, Young MJ, Schwartz PH. Klassen H, et al. J Neurosci Res. 2004 Aug 1;77(3):334-43. doi: 10.1002/jnr.20183. J Neurosci Res. 2004. PMID: 15248289 - Induction of functional photoreceptor phenotype by exogenous Crx expression in mouse retinal stem cells.
Jomary C, Jones SE. Jomary C, et al. Invest Ophthalmol Vis Sci. 2008 Jan;49(1):429-37. doi: 10.1167/iovs.07-0812. Invest Ophthalmol Vis Sci. 2008. PMID: 18172122 - Progenitor cells from the porcine neural retina express photoreceptor markers after transplantation to the subretinal space of allorecipients.
Klassen H, Kiilgaard JF, Zahir T, Ziaeian B, Kirov I, Scherfig E, Warfvinge K, Young MJ. Klassen H, et al. Stem Cells. 2007 May;25(5):1222-30. doi: 10.1634/stemcells.2006-0541. Epub 2007 Jan 11. Stem Cells. 2007. PMID: 17218397 - Imaging techniques in retinal research.
Morgan J, Huckfeldt R, Wong RO. Morgan J, et al. Exp Eye Res. 2005 Mar;80(3):297-306. doi: 10.1016/j.exer.2004.12.010. Exp Eye Res. 2005. PMID: 15721612 Review.
Cited by
- End binding-3 inhibitor activates regenerative program in age-related macular degeneration.
Lee Q, Chan WC, Qu X, Sun Y, Abdelkarim H, Le J, Saqib U, Sun MY, Kruse K, Banerjee A, Hitchinson B, Geyer M, Huang F, Guaiquil V, Mutso AA, Sanders M, Rosenblatt MI, Maienschein-Cline M, Lawrence MS, Gaponenko V, Malik AB, Komarova YA. Lee Q, et al. Cell Rep Med. 2023 Oct 17;4(10):101223. doi: 10.1016/j.xcrm.2023.101223. Epub 2023 Oct 3. Cell Rep Med. 2023. PMID: 37794584 Free PMC article. - Polarized RPE Secretome Preserves Photoreceptors in Retinal Dystrophic RCS Rats.
Ahluwalia K, Martinez-Camarillo JC, Thomas BB, Naik A, Gonzalez-Calle A, Pollalis D, Lebkowski J, Lee SY, Mitra D, Louie SG, Humayun MS. Ahluwalia K, et al. Cells. 2023 Jun 22;12(13):1689. doi: 10.3390/cells12131689. Cells. 2023. PMID: 37443724 Free PMC article. - Neural differentiation of human retinal pigment epithelial cells on alginate/gelatin substrate.
Shamsnajafabadi H, Soheili ZS, Samiee S, Ahmadieh H, Pirmardan ER, Haghighi M. Shamsnajafabadi H, et al. Mol Vis. 2022 Dec 12;28:412-431. eCollection 2022. Mol Vis. 2022. PMID: 36601411 Free PMC article. - Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision.
Karamali F, Behtaj S, Babaei-Abraki S, Hadady H, Atefi A, Savoj S, Soroushzadeh S, Najafian S, Nasr Esfahani MH, Klassen H. Karamali F, et al. J Transl Med. 2022 Dec 7;20(1):572. doi: 10.1186/s12967-022-03738-4. J Transl Med. 2022. PMID: 36476500 Free PMC article. Review. - Advances in cell therapies using stem cells/progenitors as a novel approach for neurovascular repair of the diabetic retina.
Lechner J, Medina RJ, Lois N, Stitt AW. Lechner J, et al. Stem Cell Res Ther. 2022 Jul 30;13(1):388. doi: 10.1186/s13287-022-03073-x. Stem Cell Res Ther. 2022. PMID: 35907890 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous