DAI/ZBP1 recruits RIP1 and RIP3 through RIP homotypic interaction motifs to activate NF-kappaB - PubMed (original) (raw)

DAI/ZBP1 recruits RIP1 and RIP3 through RIP homotypic interaction motifs to activate NF-kappaB

Manuele Rebsamen et al. EMBO Rep. 2009 Aug.

Abstract

Detection of viral nucleic acids is central to antiviral immunity. Recently, DAI/ZBP1 (DNA-dependent activator of IRFs/Z-DNA binding protein 1) was identified as a cytoplasmic DNA sensor and shown to activate the interferon regulatory factor (IRF) and nuclear factor-kappa B (NF-kappaB) transcription factors, leading to type-I interferon production. DAI-induced IRF activation depends on TANK-binding kinase 1 (TBK1), whereas signalling pathways and molecular components involved in NF-kappaB activation remain elusive. Here, we report the identification of two receptor-interacting protein (RIP) homotypic interaction motifs (RHIMs) in the DAI protein sequence, and show that these domains relay DAI-induced NF-kappaB signals through the recruitment of the RHIM-containing kinases RIP1 and RIP3. We show that knockdown of not only RIP1, but also RIP3 affects DAI-induced NF-kappaB activation. Importantly, RIP recruitment to DAI is inhibited by the RHIM-containing murine cytomegalovirus (MCMV) protein M45. These findings delineate the DAI signalling pathway to NF-kappaB and suggest a possible new immune modulation strategy of the MCMV.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1

Figure 1

DAI/ZBP1 contains two RHIM domains that mediate the recruitment of RIP1 and RIP3. (A) Sequence alignment of the RHIM domains of human and murine RIP1, RIP3, TRIF and DAI. The four amino-acid motif crucial for RHIM function is boxed. The black and grey shading indicate more than 60% amino-acid-sequence identity and similarity, respectively. (B,C,E,F,H,I) HEK293T cells were transfected with the indicated VSV or Flag constructs. Immunoprecipitates and cell extracts were analysed by immunoblot. In (F), white and black arrowheads indicate modified and unmodified RIP3, respectively (see text and Fig 3C–E) (D) Domain architecture of RIP1 and RIP3, and schematic views of deletion constructs used in (C), (E) and (F). RHIM* denotes the construct with alanine substitutions of the four amino acids highlighted in (A). (G) Domain architecture of human DAI and schematic views of the constructs used in this study. RHIM* denotes the construct with alanine substitutions of the four amino acids highlighted in (A) in the indicated RHIM domains. DAI, DNA-dependent activator of IRFs; DD, death domain; HEK, human embryonic kidney; Hs, human; IB, immunoblot; ID, intermediate domain; IP, immunoprecipitates; KD, kinase domain; Mm, murine; RHIM, RIP homotypic interaction motif; RIP, receptor-interacting protein; VSV, vesicular stomatitis virus; WT, wild type; XT, cell extracts; ZBP1, Z-DNA binding protein 1.

Figure 2

Figure 2

DAI-induced NF-κB activation is RHIM dependent. (A) HEK239T were transfected with an NF-κB reporter plasmid together with an empty plasmid or increasing doses of the indicated DAI constructs, and were analysed for NF-κB-dependent luciferase activity 24 h later. Data are the mean values±s.d. of three transfection points from one experiment; results are representative of three independent experiments. (B) HEK293T cells were transfected with the indicated Flag–DAI constructs or an empty vector. IPs and XTs were analysed for the presence of endogenous RIP1 by IB. (C) HEK293-TRex cells inducibly expressing the indicated Flag–DAI constructs or an empty vector were treated with doxycycline (Dox; 200 ng/ml) for the indicated times; XTs were analysed by IB. DAI, DNA-dependent activator of IRFs; HEK, human embryonic kidney; IB, immunoblot; IP, immunoprecipitates; NF-κB, nuclear factor-kappa B; RHIM, RIP homotypic interaction motifs; RIP, receptor-interacting protein; VSV, vesicular stomatitis virus; WT, wild type; XT, cell extracts.

Figure 3

Figure 3

DAI-induced NF-κB activation requires RIP1 and RIP3. (A) HEK293T cells were transfected with 20 nM of the indicated siRNAs and, 36 h later, re-transfected with an NF-κB reporter plasmid together with an empty plasmid or two doses of DAI. NF-κB-dependent luciferase activity was measured 24 h after the second transfection. Data are the mean values±s.d. of three transfection points from one experiment; results are representative of three independent experiments. (B,C) HEK293T cells were transfected with VSV–DAI or an empty vector and the indicated Flag constructs. IP and XTs were analysed by IB. In (B), increasing doses of plasmid are shown. (D) HEK293T cells were transfected with Flag–RIP3 or an empty vector and the indicated VSV constructs. At 24 h after transfection, cell lysates were incubated with or without CIP (10 U per 30 μl, 10 min, 37°C) and analysed by IB. (E) HEK293T cells were transfected with the indicated constructs and analysed by IB. In (C–E), white and black arrowheads indicate modified and unmodified RIP3, respectively. CIP, calf intestinal alkaline phosphatase; DAI, DNA-dependent activator of IRFs; HEK, human embryonic kidney; IB, immunoblot; IP, immunoprecipitates; NF-κB, nuclear factor-kappa B; RHIM, RIP homotypic interaction motifs; RIP, receptor-interacting protein; siRNA, small interfering RNA; XT, cell extracts; VSV, vesicular stomatitis virus; WT, wild type.

Figure 4

Figure 4

The RHIM-containing protein M45 of the MCMV inhibits DAI signalling. (A) HEK293T cells were transfected with VSV–M451−277 or an empty vector and the indicated Flag constructs. IP and XT were analysed by IB. (B) HEK239T were transfected with an NF-κB reporter plasmid together with an empty plasmid (e.v) or DAI in combination with increasing doses of the indicated M45 constructs (shown in supplementary Fig S6B online) and were analysed for NF-κB-dependent luciferase activity 24 h later. Data are the mean values±s.d. of three transfection points from one experiment; results are representative of two independent experiments. (C,D) HEK293T cells were transfected with the indicated VSV or Flag constructs. IP and XT were analysed by IB. In (D), white and black arrowheads indicate modified and unmodified RIP3, respectively. The asterisks indicate constructs with alanine substitutions of the four amino acids (highlighted in supplementary Fig S6A online). DAI, DNA-dependent activator of IRFs; FL, full length; HEK, human embryonic kidney; IB, immunoblot; IP, immunoprecipitates; MCMV, murine cytomegalovirus; NF-κB, nuclear factor-kappa B; RHIM, RIP homotypic interaction motifs; RIP, receptor-interacting protein; XT, cell extracts; VSV, vesicular stomatitis virus.

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