Expression of the HER-2/neu proto-oncogene in normal human adult and fetal tissues - PubMed (original) (raw)
Affiliations
- PMID: 1973830
Expression of the HER-2/neu proto-oncogene in normal human adult and fetal tissues
M F Press et al. Oncogene. 1990 Jul.
Abstract
The HER-2/neu proto-oncogene is homologous with, but distinct from, the epidermal growth factor receptor. Current evidence indicates that this gene is frequently amplified and/or overexpressed in some human breast and ovarian cancers and that these alterations may be clinically important; however, little is known about the expression pattern of the gene in normal tissues. Using immunohistochemistry and northern blot analyses to identify the HER-2/neu protein and transcript respectively, we have evaluated a variety of normal adult and fetal tissues for HER-2/neu expression. HER-2/neu protein was identified on cell membranes of epithelial cells in the gastro-intestinal, respiratory, reproductive, and urinary tract as well as in the skin, breast and placenta. Northern hybridization confirmed the presence of the 4.5 kb transcript encoding the protein in these tissues. The amount of HER-2/neu message and protein was generally higher in fetal tissues than in the corresponding normal adult tissues. HER-2/neu expression levels in these normal tissues were similar to the levels found in non-amplified, non-overexpressing breast cancers and breast cancer cell lines. Southern hybridization of extracted DNA showed that none of the normal tissues expressing HER-2/neu had amplification of the gene. These results confirm that HER-2/neu is normally a membrane constituent of a variety of epithelial cell types.
Similar articles
- Her-2/neu expression in node-negative breast cancer: direct tissue quantitation by computerized image analysis and association of overexpression with increased risk of recurrent disease.
Press MF, Pike MC, Chazin VR, Hung G, Udove JA, Markowicz M, Danyluk J, Godolphin W, Sliwkowski M, Akita R, et al. Press MF, et al. Cancer Res. 1993 Oct 15;53(20):4960-70. Cancer Res. 1993. PMID: 8104689 - Expression pattern of the neu (NGL) gene-encoded growth factor receptor protein (p185neu) in normal and transformed epithelial tissues of the digestive tract.
Cohen JA, Weiner DB, More KF, Kokai Y, Williams WV, Maguire HC Jr, LiVolsi VA, Greene MI. Cohen JA, et al. Oncogene. 1989 Jan;4(1):81-8. Oncogene. 1989. PMID: 2563577 - The tumor suppression activity of E1A in HER-2/neu-overexpressing breast cancer.
Chang JY, Xia W, Shao R, Sorgi F, Hortobagyi GN, Huang L, Hung MC. Chang JY, et al. Oncogene. 1997 Feb 6;14(5):561-8. doi: 10.1038/sj.onc.1200861. Oncogene. 1997. PMID: 9053854 - The role of the HER-2/neu oncogene in gynecologic cancers.
Cirisano FD, Karlan BY. Cirisano FD, et al. J Soc Gynecol Investig. 1996 May-Jun;3(3):99-105. J Soc Gynecol Investig. 1996. PMID: 8796816 Review. - HER-2/neu (c-erb-B2) gene and protein in breast cancer.
Ross JS, Fletcher JA. Ross JS, et al. Am J Clin Pathol. 1999 Jul;112(1 Suppl 1):S53-67. Am J Clin Pathol. 1999. PMID: 10396301 Review.
Cited by
- Revisiting HER2 in Prostate Cancer from an Inclusive Perspective: From Biomarkers to Omics.
Mavingire N, Moore JC, Johnson JR, Dwead AM, Cropp CD, Mechref Y, Kobeissy F, Rais-Bahrami S, Woods-Burnham L. Mavingire N, et al. Cancers (Basel). 2024 Sep 25;16(19):3262. doi: 10.3390/cancers16193262. Cancers (Basel). 2024. PMID: 39409883 Free PMC article. Review. - The relevance of tumor target expression levels on IgA-mediated cytotoxicity in cancer immunotherapy.
Chan C, Cabanes NC, Jansen JHM, Guillaume J, Nederend M, Passchier EM, Gómez-Mellado VE, Peipp M, Boes M, van Tetering G, Leusen JHW. Chan C, et al. Cancer Immunol Immunother. 2024 Oct 3;73(12):238. doi: 10.1007/s00262-024-03824-0. Cancer Immunol Immunother. 2024. PMID: 39358557 Free PMC article. - HER2-low gastric cancer: is the subgroup targetable?
Shimozaki K, Fukuoka S, Ooki A, Yamaguchi K. Shimozaki K, et al. ESMO Open. 2024 Sep;9(9):103679. doi: 10.1016/j.esmoop.2024.103679. Epub 2024 Aug 22. ESMO Open. 2024. PMID: 39178538 Free PMC article. Review. - HER2-CD3-Fc Bispecific Antibody-Encoding mRNA Delivered by Lipid Nanoparticles Suppresses HER2-Positive Tumor Growth.
Hu L, Zhang S, Sienkiewicz J, Zhou H, Berahovich R, Sun J, Li M, Ocampo A, Liu X, Huang Y, Harto H, Xu S, Golubovskaya V, Wu L. Hu L, et al. Vaccines (Basel). 2024 Jul 21;12(7):808. doi: 10.3390/vaccines12070808. Vaccines (Basel). 2024. PMID: 39066446 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous