Isolation of soluble tyrosinase from human melanoma cells - PubMed (original) (raw)
Affiliations
- PMID: 1977251
Isolation of soluble tyrosinase from human melanoma cells
A Wittbjer et al. Acta Derm Venereol. 1990.
Abstract
In the human melanoma cell tyrosinase exists in a membraneous and a soluble form. The membraneous enzyme has an N-terminal amino acid sequence identical to that predicted from a human c-DNA clone by Kwon et al.. The soluble form has now been isolated by a technique mainly based on the trypsin resistence of the enzyme and the use of hydrophobic interaction chromatography. The specific dopa oxidase activity of the soluble enzyme was 300 mumol/min x mg protein. On isoelectric focusing the enzyme was found in at least ten bands, pI between 3.8-4.6. The molecular weight was found to be 53,000 D. The N-terminal amino acid sequence was the same as that found in the membrane bound form of the enzyme, i.e. the protein maps at the c-albino locus.
Similar articles
- Isolation of tyrosinase from bovine eyes.
Wittbjer A, Odh G, Rosengren AM, Rosengren E, Rorsman H. Wittbjer A, et al. Pigment Cell Res. 1990 Sep;3(3):168-72. doi: 10.1111/j.1600-0749.1990.tb00283.x. Pigment Cell Res. 1990. PMID: 2127099 - Isolation of human tyrosinase from cultured melanoma cells.
Wittbjer A, Dahlbäck B, Odh G, Rosengren AM, Rosengren E, Rorsman H. Wittbjer A, et al. Acta Derm Venereol. 1989;69(2):125-31. Acta Derm Venereol. 1989. PMID: 2564229 - Molecular basis for the heterogeneity of human tyrosinase.
Shibahara S, Tomita Y, Tagami H, Müller RM, Cohen T. Shibahara S, et al. Tohoku J Exp Med. 1988 Dec;156(4):403-14. doi: 10.1620/tjem.156.403. Tohoku J Exp Med. 1988. PMID: 2854305 - Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus.
Kwon BS, Haq AK, Pomerantz SH, Halaban R. Kwon BS, et al. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7473-7. doi: 10.1073/pnas.84.21.7473. Proc Natl Acad Sci U S A. 1987. PMID: 2823263 Free PMC article. - Molecular and biological control of melanogenesis through tyrosinase genes and intrinsic and extrinsic regulatory factors.
Mishima Y. Mishima Y. Pigment Cell Res. 1994 Dec;7(6):376-87. doi: 10.1111/j.1600-0749.1994.tb00065.x. Pigment Cell Res. 1994. PMID: 7761345 Review.
Cited by
- Purification of Recombinant Human Tyrosinase from Insect Larvae Infected with the Baculovirus Vector.
Dolinska MB, Wingfield PT, Sergeev YV. Dolinska MB, et al. Curr Protoc Protein Sci. 2017 Aug 1;89:6.15.1-6.15.12. doi: 10.1002/cpps.37. Curr Protoc Protein Sci. 2017. PMID: 28762492 Free PMC article. - Aqueous humor tyrosinase activity is indicative of iris melanocyte toxicity.
Mahanty S, Kawali AA, Dakappa SS, Mahendradas P, Kurian M, Kharbanda V, Shetty R, Setty SRG. Mahanty S, et al. Exp Eye Res. 2017 Sep;162:79-85. doi: 10.1016/j.exer.2017.07.006. Epub 2017 Jul 13. Exp Eye Res. 2017. PMID: 28712540 Free PMC article. - Albinism-causing mutations in recombinant human tyrosinase alter intrinsic enzymatic activity.
Dolinska MB, Kovaleva E, Backlund P, Wingfield PT, Brooks BP, Sergeev YV. Dolinska MB, et al. PLoS One. 2014 Jan 2;9(1):e84494. doi: 10.1371/journal.pone.0084494. eCollection 2014. PLoS One. 2014. PMID: 24392141 Free PMC article. - The clinical significance of antityrosinase antibodies in melanoma and related hypopigmentary lesions.
Merimsky O, Shoenfeld Y, Fishman P. Merimsky O, et al. Clin Rev Allergy Immunol. 1998 Fall;16(3):227-36. doi: 10.1007/BF02737633. Clin Rev Allergy Immunol. 1998. PMID: 9773250 Review. - Aberrant retention of tyrosinase in the endoplasmic reticulum mediates accelerated degradation of the enzyme and contributes to the dedifferentiated phenotype of amelanotic melanoma cells.
Halaban R, Cheng E, Zhang Y, Moellmann G, Hanlon D, Michalak M, Setaluri V, Hebert DN. Halaban R, et al. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6210-5. doi: 10.1073/pnas.94.12.6210. Proc Natl Acad Sci U S A. 1997. PMID: 9177196 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous