Specific cis-acting sequence for PHO8 expression interacts with PHO4 protein, a positive regulatory factor, in Saccharomyces cerevisiae - PubMed (original) (raw)
Specific cis-acting sequence for PHO8 expression interacts with PHO4 protein, a positive regulatory factor, in Saccharomyces cerevisiae
N Hayashi et al. Mol Cell Biol. 1991 Feb.
Abstract
The PHO8 gene of Saccharomyces cerevisiae encodes repressible alkaline phosphatase (rALPase; EC 3.1.3.1). The rALPase activity of the cells is two to three times higher in medium containing a low concentration of Pi than in high-Pi medium due to transcription of PHO8. The Pi signals are conveyed to PHO8 by binding of PHO4 protein, a positive regulatory factor, to a promoter region of PHO8 (PHO8p) under the influence of the PHO regulatory circuit. Deletion analysis of PHO8p DNA revealed two separate regulatory regions required for derepression of rALPase located at nucleotide positions -704 to -661 (distal region) and -548 to -502 (proximal region) and an inhibitory region located at -421 to -289 relative to the translation initiation codon. Gel retardation experiments showed that a beta-galactosidase-PHO4 fusion protein binds to a 132-bp PHO8p fragment bearing the proximal region but not to a 226-bp PHO8 DNA bearing the distal region. The fusion protein also binds to a synthetic oligonucleotide having the same 12-bp nucleotide sequence as the PHO8p DNA from positions -536 to -525. The 132-bp PHO8p fragment, connected at position -281 of the 5' upstream region of a HIS5'-'lacZ fused gene, could sense Pi signals in vivo, but a 20-bp synthetic oligonucleotide having the same sequence from -544 to -525 of the PHO8p DNA could not. Linker insertions in the PHO8p DNA indicated that the 5-bp sequence 5'-CACGT-3' from positions -535 to -531 is essential for binding the beta-galactosidase-PHO4 fusion protein and for derepression of rALPase.
Similar articles
- Activation of the weakly regulated PHO8 promoter in S. cerevisiae: chromatin transition and binding sites for the positive regulatory protein PHO4.
Barbarić S, Fascher KD, Hörz W. Barbarić S, et al. Nucleic Acids Res. 1992 Mar 11;20(5):1031-8. doi: 10.1093/nar/20.5.1031. Nucleic Acids Res. 1992. PMID: 1567507 Free PMC article. - Structural characteristics of the PHO8 gene encoding repressible alkaline phosphatase in Saccharomyces cerevisiae.
Kaneko Y, Hayashi N, Toh-e A, Banno I, Oshima Y. Kaneko Y, et al. Gene. 1987;58(1):137-48. doi: 10.1016/0378-1119(87)90036-9. Gene. 1987. PMID: 3319783 - Transcriptional and post-transcriptional control of PHO8 expression by PHO regulatory genes in Saccharomyces cerevisiae.
Kaneko Y, Tamai Y, Toh-e A, Oshima Y. Kaneko Y, et al. Mol Cell Biol. 1985 Jan;5(1):248-52. doi: 10.1128/mcb.5.1.248-252.1985. Mol Cell Biol. 1985. PMID: 2984552 Free PMC article. - The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions.
Vogel K, Hörz W, Hinnen A. Vogel K, et al. Mol Cell Biol. 1989 May;9(5):2050-7. doi: 10.1128/mcb.9.5.2050-2057.1989. Mol Cell Biol. 1989. PMID: 2664469 Free PMC article. - The phosphatase system in Saccharomyces cerevisiae.
Oshima Y. Oshima Y. Genes Genet Syst. 1997 Dec;72(6):323-34. doi: 10.1266/ggs.72.323. Genes Genet Syst. 1997. PMID: 9544531 Review.
Cited by
- Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
Lieleg C, Novacic A, Musladin S, Schmid A, Akpinar GG, Barbaric S, Korber P. Lieleg C, et al. Int J Mol Sci. 2023 Mar 3;24(5):4949. doi: 10.3390/ijms24054949. Int J Mol Sci. 2023. PMID: 36902382 Free PMC article. - Phosphate-responsive promoter of a Pichia pastoris sodium phosphate symporter.
Ahn J, Hong J, Park M, Lee H, Lee E, Kim C, Lee J, Choi ES, Jung JK, Lee H. Ahn J, et al. Appl Environ Microbiol. 2009 Jun;75(11):3528-34. doi: 10.1128/AEM.02913-08. Epub 2009 Mar 27. Appl Environ Microbiol. 2009. PMID: 19329662 Free PMC article. - Discovering novel cis-regulatory motifs using functional networks.
Ettwiller LM, Rung J, Birney E. Ettwiller LM, et al. Genome Res. 2003 May;13(5):883-95. doi: 10.1101/gr.866403. Genome Res. 2003. PMID: 12727907 Free PMC article. - Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, Oshima Y, Hakoshima T. Shimizu T, et al. EMBO J. 1997 Aug 1;16(15):4689-97. doi: 10.1093/emboj/16.15.4689. EMBO J. 1997. PMID: 9303313 Free PMC article. - Structure and distribution of specific cis-elements for transcriptional regulation of PHO84 in Saccharomyces cerevisiae.
Ogawa N, Saitoh H, Miura K, Magbanua JP, Bun-ya M, Harashima S, Oshima Y. Ogawa N, et al. Mol Gen Genet. 1995 Dec 10;249(4):406-16. doi: 10.1007/BF00287102. Mol Gen Genet. 1995. PMID: 8552045
References
- Cell. 1985 Dec;43(2 Pt 1):439-48 - PubMed
- Methods Enzymol. 1980;65(1):499-560 - PubMed
- Mol Cell Biol. 1985 Aug;5(8):2131-41 - PubMed
- EMBO J. 1986 Oct;5(10):2689-96 - PubMed
- Mol Cell Biol. 1986 Jul;6(7):2490-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous