Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus - PubMed (original) (raw)
Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus
M M Lai et al. Proc Natl Acad Sci U S A. 1977 Nov.
Abstract
The genome size of 20 transformation-defective (td) viruses derived from different strains of Rous sarcoma viruses [Prague (subgroups A and C), Schmidt-Ruppin (subgroups A and D) (SR-D), Bratislava 77, and Carr-Zilber subgroup D)] was examined by polyacrylamide gel electrophoresis. All of the td viruses except td SR-D have 35S RNA of the same size-i.e., class b RNA. Two of five td SR-D viruses examined have a slightly larger RNA, corresponding to a td deletion that is about 25% smaller than that of class b RNA. However, the RNase T(1)-oligonucleotide fingerprints of all the td SR-D viruses are identical, lacking two sarcoma-specific oligonucleotides. The fingerprints of these viruses also showed a minor oligonucleotide present at very low concentration. A study of heteroduplex molecules formed between genome-length cDNA made from wild-type SR-D and 35S RNA of td SR-D showed a deletion loop of 2.0 and 1.5 kilobases, respectively, at the map position of the src gene for these two classes of td SR-D viruses, confirming the results of polyacrylamide gel electrophoresis. In addition, some heteroduplex molecules with a substitution loop of 0.6-0.7 kilobase at the same site as the deletion loop were observed in all five of the td SR-D viruses. We conclude that some of the td SR-D viruses have a partially deleted src gene and that all of the td SR-D viruses have incorporated heterologous sequences of distinct length in some RNA molecules at the position of the src gene. The nature and origin of these heterologous sequences are discussed.
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References
- Nucleic Acids Res. 1977;4(5):1539-52 - PubMed
- Proc Natl Acad Sci U S A. 1977 Feb;74(2):477-81 - PubMed
- Nucleic Acids Res. 1976 Jul;3(7):1647-58 - PubMed
- Curr Top Microbiol Immunol. 1976;75:111-52 - PubMed
- Virology. 1973 Jul;54(1):207-19 - PubMed
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