Nucleotide-induced global conformational changes of flagellar dynein arms revealed by in situ analysis - PubMed (original) (raw)

doi: 10.1038/nsmb.1832. Epub 2010 May 9.

Affiliations

• PMID: 20453857
• DOI: 10.1038/nsmb.1832

Nucleotide-induced global conformational changes of flagellar dynein arms revealed by in situ analysis

Tandis Movassagh et al. Nat Struct Mol Biol. 2010 Jun.

Abstract

Outer and inner dynein arms generate force for the flagellar/ciliary bending motion. Although nucleotide-induced structural change of dynein heavy chains (the ATP-driven motor) was proven in vitro, our lack of knowledge in situ has precluded an understanding of the bending mechanism. Here we reveal nucleotide-induced global structural changes of the outer and inner dynein arms of Chlamydomonas reinhardtii flagella in situ using electron cryotomography. The ATPase domains of the dynein heavy chains move toward the distal end, and the N-terminal tail bends sharply during product release. This motion could drive the adjacent microtubule to cause a sliding motion. In contrast to in vitro results, in the presence of nucleotides, outer dynein arms coexist as clusters of apo or nucleotide-bound forms in situ. This implies a cooperative switching, which may be related to the mechanism of bending.

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Comment in

• Axonemal dyneins winch the cilium.
  King SM. King SM. Nat Struct Mol Biol. 2010 Jun;17(6):673-4. doi: 10.1038/nsmb0610-673. Nat Struct Mol Biol. 2010. PMID: 20520659 No abstract available.

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References

1. 1. J Cell Biol. 2009 Aug 10;186(3):437-46 - PubMed
2. 1. Cell. 2009 Feb 6;136(3):485-95 - PubMed
3. 1. Int Rev Cytol. 2002;219:115-55 - PubMed
4. 1. J Mol Biol. 2007 May 18;368(5):1249-58 - PubMed
5. 1. Cell Motil Cytoskeleton. 2005 Oct;62(2):69-83 - PubMed

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