Reduction to homozygosity involving p53 in esophageal cancers demonstrated by the polymerase chain reaction - PubMed (original) (raw)
Reduction to homozygosity involving p53 in esophageal cancers demonstrated by the polymerase chain reaction
S J Meltzer et al. Proc Natl Acad Sci U S A. 1991.
Abstract
Loss of heterozygosity affecting chromosome 17p has been detected at high frequencies in a variety of human tumors, including cancers of the colon, breast, lung, and brain. One presumed target of these losses is p53, a tumor suppressor gene located on 17p. To our knowledge, loss of heterozygosity has not yet been reported at any locus, including p53, in human esophageal cancer. Moreover, current methods of detecting loss of heterozygosity depend on the availability of large amounts of high molecular weight DNA, making the study of small biopsy specimens or paraffin-embedded tissues problematic. We examined 52 primary human esophageal neoplasms for loss of heterozygosity affecting the p53 gene by using the polymerase chain reaction. Loss of one allele was detected in 52% of informative cases and was more common in squamous carcinomas than in adenocarcinomas. Southern blot analysis was used to confirm polymerase chain reaction-derived data. The identification of allelic loss in approximately half of the tumors analyzed supports the hypothesis that inactivation of p53 is involved in the pathogenesis of esophageal cancer.
Similar articles
- Loss of heterozygosity involves multiple tumor suppressor genes in human esophageal cancers.
Huang Y, Boynton RF, Blount PL, Silverstein RJ, Yin J, Tong Y, McDaniel TK, Newkirk C, Resau JH, Sridhara R, Reid BJ, Meltzer SJ. Huang Y, et al. Cancer Res. 1992 Dec 1;52(23):6525-30. Cancer Res. 1992. PMID: 1423299 - Frequent mutation of the p53 gene in human esophageal cancer.
Hollstein MC, Metcalf RA, Welsh JA, Montesano R, Harris CC. Hollstein MC, et al. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9958-61. doi: 10.1073/pnas.87.24.9958. Proc Natl Acad Sci U S A. 1990. PMID: 2263646 Free PMC article. - Molecular genetic studies of tumor suppressor gene regions on chromosomes 13 and 17 in colorectal tumors.
Lothe RA, Fossli T, Danielsen HE, Stenwig AE, Nesland JM, Gallie B, Børresen AL. Lothe RA, et al. J Natl Cancer Inst. 1992 Jul 15;84(14):1100-8. doi: 10.1093/jnci/84.14.1100. J Natl Cancer Inst. 1992. PMID: 1619684 - Analysis of chromosome 22 loci in meningioma. Alterations in the leukemia inhibitory factor (LIF) locus.
Pergolizzi RG, Erster SH. Pergolizzi RG, et al. Mol Chem Neuropathol. 1994 Feb-Apr;21(2-3):189-217. doi: 10.1007/BF02815351. Mol Chem Neuropathol. 1994. PMID: 7916188 Review. - Representational difference analysis as a tool in the search for new tumor suppressor genes.
Hollestelle A, Schutte M. Hollestelle A, et al. Methods Mol Med. 2005;103:143-59. doi: 10.1385/1-59259-780-7:143. Methods Mol Med. 2005. PMID: 15542904 Review.
Cited by
- p53 protein accumulation in oesophageal squamous cell carcinomas and precancerous lesions.
Volant A, Nousbaum JB, Giroux MA, Roué-Quintin I, Metges JP, Férec C, Gouérou H, Robaszkiewicz M. Volant A, et al. J Clin Pathol. 1995 Jun;48(6):531-4. doi: 10.1136/jcp.48.6.531. J Clin Pathol. 1995. PMID: 7665695 Free PMC article. - Modulation of oncogene and tumor-suppressor gene expression: a novel strategy for cancer prevention and treatment.
Roth JA. Roth JA. Ann Surg Oncol. 1994 Jan;1(1):79-86. doi: 10.1007/BF02303545. Ann Surg Oncol. 1994. PMID: 7834433 Review. - NSAIDs modulate CDKN2A, TP53, and DNA content risk for progression to esophageal adenocarcinoma.
Galipeau PC, Li X, Blount PL, Maley CC, Sanchez CA, Odze RD, Ayub K, Rabinovitch PS, Vaughan TL, Reid BJ. Galipeau PC, et al. PLoS Med. 2007 Feb;4(2):e67. doi: 10.1371/journal.pmed.0040067. PLoS Med. 2007. PMID: 17326708 Free PMC article. - Clonal ordering of 17p and 5q allelic losses in Barrett dysplasia and adenocarcinoma.
Blount PL, Meltzer SJ, Yin J, Huang Y, Krasna MJ, Reid BJ. Blount PL, et al. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3221-5. doi: 10.1073/pnas.90.8.3221. Proc Natl Acad Sci U S A. 1993. PMID: 8475062 Free PMC article. - Intraperitoneal dedifferentiated liposarcoma: a case report.
Karaman A, Kabalar ME, Ozcan O, Koca T, Binici DN. Karaman A, et al. World J Gastroenterol. 2008 Oct 14;14(38):5927-9. doi: 10.3748/wjg.14.5927. World J Gastroenterol. 2008. PMID: 18855997 Free PMC article.
References
- Mol Cell Biol. 1991 Jan;11(1):12-9 - PubMed
- Cancer Res. 1990 Jun 15;50(12):3627-30 - PubMed
- Cell Growth Differ. 1990 Dec;1(12):571-80 - PubMed
- Cancer Res. 1991 Apr 15;51(8):2113-7 - PubMed
- Nature. 1985 Mar 7-13;314(6006):67-73 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous