Genome-wide profiling of interleukin-4 and STAT6 transcription factor regulation of human Th2 cell programming - PubMed (original) (raw)

. 2010 Jun 25;32(6):852-62.

doi: 10.1016/j.immuni.2010.06.011.

Henna Järvenpää, Soile Tuomela, Sunil Raghav, Helena Ahlfors, Kirsti Laurila, Bhawna Gupta, Riikka J Lund, Johanna Tahvanainen, R David Hawkins, Matej Oresic, Harri Lähdesmäki, Omid Rasool, Kanury V Rao, Tero Aittokallio, Riitta Lahesmaa

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Genome-wide profiling of interleukin-4 and STAT6 transcription factor regulation of human Th2 cell programming

Laura L Elo et al. Immunity. 2010.

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Abstract

Dissecting the molecular mechanisms by which T helper (Th) cells differentiate to effector Th2 cells is important for understanding the pathogenesis of immune-mediated diseases, such as asthma and allergy. Because the STAT6 transcription factor is an upstream mediator required for interleukin-4 (IL-4)-induced Th2 cell differentiation, its targets include genes important for this process. Using primary human CD4(+) T cells, and by blocking STAT6 with RNAi, we identified a number of direct and indirect targets of STAT6 with ChIP sequencing. The integration of these data sets with detailed kinetics of IL-4-driven transcriptional changes showed that STAT6 was predominantly needed for the activation of transcription leading to the Th2 cell phenotype. This integrated genome-wide data on IL-4- and STAT6-mediated transcription provide a unique resource for studies on Th cell differentiation and, in particular, for designing interventions of human Th2 cell responses.

Copyright 2010 Elsevier Inc. All rights reserved.

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