The TRPC Family of Ion Channels: Relation to the TRP Superfamily and Role in Receptor- and Store-Operated Calcium Entry - PubMed (original) (raw)

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In: TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades. Boca Raton (FL): CRC Press/Taylor & Francis; 2007. Chapter 1.

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The TRPC Family of Ion Channels: Relation to the TRP Superfamily and Role in Receptor- and Store-Operated Calcium Entry

Joel Abramowitz et al.

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The Drosophila trp mutation is responsible for the phenotype called transient receptor potential, an alteration of the fly’s electrorentinogram in which its sustained phase is missing [1,2]. The responsible gene was cloned in 1989 [3]. Its amino acid sequence predicted a protein with eight hydrophobic segments that could potentially form transmembrane segments. Purification and cloning of a calmodulin-binding protein from Drosophila heads showed it to be a homologue of trp. It received the name trp-like or trpl [4]. Its discoverers highlighted the existence of limited sequence similarities between trp/trpl and voltage-sensitive Na+ and Ca2+ channels. Expression of trpl in silkworm cells of Spodoptera frugiperda (Sf9 cells) did indeed lead to appearance of cation channels [5]. In keeping with both a role for trp and trpl in insect phototransduction, and the fact that insect phototransduction is biochemically akin to mammalian signal transduction based on the Gq-PLC pathway instead of a transducin-phosphodiesterase (Gt-PDE) pathway [6,7], the trpl channels expressed in Sf9 cells could be activated by a Gq-coupled GPCR [8].

Copyright © 2007, Taylor & Francis Group, LLC.

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