The acquisition of antigen cross-presentation function by newly formed dendritic cells - PubMed (original) (raw)

. 2011 May 1;186(9):5184-92.

doi: 10.4049/jimmunol.1002683. Epub 2011 Mar 21.

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The acquisition of antigen cross-presentation function by newly formed dendritic cells

Priyanka Sathe et al. J Immunol. 2011.

Abstract

The development of Ag-presenting functions by murine dendritic cells (DCs) of the CD8(+) DC lineage was studied using a Flt-3 ligand stimulated bone-marrow culture system. Although newly formed DCs of this lineage are capable of Ag uptake and efficient presentation to T cells on MHC class II, they initially lack the ability to cross-present exogenous Ags on MHC class I. Cross-presentation capacity is acquired as a subsequent maturation step, promoted by cytokines such as GM-CSF. The development of cross-presentation capacity by the DCs in these cultures may be monitored by the parallel development of DC surface expression of CD103. However, the expression of CD103 and cross-presentation capacity are not always linked; therefore, CD103 is not an essential part of the cross-presentation machinery. These results explain the considerable variability in CD103 expression by CD8(+) DCs as well as the findings that not all DCs of this lineage are capable of cross-presentation.

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