Knockout of 5-lipoxygenase results in age-dependent anxiety-like behavior in female mice - PubMed (original) (raw)

Knockout of 5-lipoxygenase results in age-dependent anxiety-like behavior in female mice

Yash B Joshi et al. PLoS One. 2011.

Abstract

Background: The enzyme 5-lipoxygenase (5LO) has been implicated in a variety of neurological and psychiatric disorders including anxiety. Knockout of 5LO has previously been shown to alter anxiety-like behavior in mice at a young age but the effect of 5LO knockout on older animals has not been characterized.

Methodology/principal findings: Here we used the elevated plus maze behavioral paradigm to measure anxiety-like behavior in female mice lacking 5LO (5LO-KO) at three different ages. Adolescent 5LO-KO animals did not significantly differ from wild-type (WT) animals in anxiety-like behavior. However, adult and older mice exhibited increased anxiety-like behavior compared to WT controls.

Conclusions: These results indicate that 5LO plays a role in the development of the anxiety-like phenotype in an age-dependent manner in female mice. Future work should further investigate this interaction as 5LO may prove to be an important molecular target for the development of novel anxiolytic therapies.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1

Figure 1. Elevated plus maze behavior in 3 month-old mice.

Percentage of total entries into the closed arm (A), open arm (B), total arm entries (C), percentage of total time spent in closed (D) and open arm (E). No difference was detected in any parameter between wild type (WT) (open bars) and 5LO-KO mice (closed bars) (WT, n = 6; 5LO-KO n = 3).

Figure 2

Figure 2. Elevated plus maze behavior in 6 mo old mice.

Percentage of total entries into the closed arm (A), open arm (B), total arm entries (C) and percentage of total time spent in closed (D) and open arm (E). 5LO-KO mice (closed bars) spent more time in the closed arm and less time in the open arm than wild type (WT) mice (open bars). 5LO-KO animals also entered less total arms than WT. (WT, n = 4; 5LO-KO, n = 4; P<0.05).

Figure 3

Figure 3. Elevated plus maze behavior in 12 mo old mice.

Percentage of total entries into the closed arm (A), open arm (B), total arm entries (C) and percentage of total time spent in closed (D) and open arm (E). 5LO-KO mice (closed arms) entered the closed arms more and entered open arms less than wild type (WT) animals (open arms). 5LO-KO animals also spent more time in the closed arm and less time in the open arm than WT mice. No differences in total arm entries were noted. (WT, n = 4, 5LO-KO, n = 6).

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