Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content - PubMed (original) (raw)
. 2014 Apr;126(8):557-66.
doi: 10.1042/CS20130295.
Affiliations
- PMID: 24215591
- DOI: 10.1042/CS20130295
Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content
Tim Snijders et al. Clin Sci (Lond). 2014 Apr.
Abstract
Muscle disuse leads to a considerable loss in skeletal muscle mass and strength. However, the cellular mechanisms underlying disuse-induced muscle fibre atrophy remain to be elucidated. Therefore we assessed the effect of muscle disuse on the CSA (cross-sectional area), muscle fibre size, satellite cell content and associated myocellular signalling pathways of the quadriceps muscle. A total of 12 healthy young (24±1 years of age) men were subjected to 2 weeks of one-legged knee immobilization via a full-leg cast. Before and immediately after the immobilization period and after 6 weeks of natural rehabilitation, muscle strength [1RM (one-repetition maximum)], muscle CSA [single slice CT (computed tomography) scan] and muscle fibre type characteristics (muscle biopsies) were assessed. Protein and/or mRNA expression of key genes [i.e. MYOD (myogenic differentiation), MYOG (myogenin) and MSTN (myostatin)] in the satellite cell regulatory pathways were determined using Western blotting and RT-PCR (real-time PCR) analyses respectively. The present study found that quadriceps CSA declined following immobilization by 8±2% (P<0.05). In agreement, both type I and type II muscle fibre size decreased 7±3% and 13±4% respectively (P<0.05). No changes were observed in satellite cell content following immobilization in either type I or type II muscle fibres. Muscle MYOG mRNA expression doubled (P<0.05), whereas MSTN protein expression decreased 30±9% (P<0.05) following immobilization. Muscle mass and strength returned to the baseline values within 6 weeks of recovery without any specific rehabilitative programme. In conclusion, 2 weeks of muscle disuse leads to considerable loss in skeletal muscle mass and strength. The loss in muscle mass was attributed to both type I and type II muscle fibre atrophy, and was not accompanied by a decline in satellite cell content.
Similar articles
- Substantial skeletal muscle loss occurs during only 5 days of disuse.
Wall BT, Dirks ML, Snijders T, Senden JM, Dolmans J, van Loon LJ. Wall BT, et al. Acta Physiol (Oxf). 2014 Mar;210(3):600-11. doi: 10.1111/apha.12190. Epub 2013 Dec 5. Acta Physiol (Oxf). 2014. PMID: 24168489 - Neuromuscular electrical stimulation prevents muscle disuse atrophy during leg immobilization in humans.
Dirks ML, Wall BT, Snijders T, Ottenbros CL, Verdijk LB, van Loon LJ. Dirks ML, et al. Acta Physiol (Oxf). 2014 Mar;210(3):628-41. doi: 10.1111/apha.12200. Epub 2013 Dec 12. Acta Physiol (Oxf). 2014. PMID: 24251881 Clinical Trial. - β-Hydroxy-β-methylbutyrate (HMB) enhances the proliferation of satellite cells in fast muscles of aged rats during recovery from disuse atrophy.
Alway SE, Pereira SL, Edens NK, Hao Y, Bennett BT. Alway SE, et al. Exp Gerontol. 2013 Sep;48(9):973-84. doi: 10.1016/j.exger.2013.06.005. Epub 2013 Jul 4. Exp Gerontol. 2013. PMID: 23832076 - Skeletal muscle atrophy during short-term disuse: implications for age-related sarcopenia.
Wall BT, Dirks ML, van Loon LJ. Wall BT, et al. Ageing Res Rev. 2013 Sep;12(4):898-906. doi: 10.1016/j.arr.2013.07.003. Epub 2013 Aug 12. Ageing Res Rev. 2013. PMID: 23948422 Review. - Skeletal muscle immobilisation-induced atrophy: mechanistic insights from human studies.
Deane CS, Piasecki M, Atherton PJ. Deane CS, et al. Clin Sci (Lond). 2024 Jun 19;138(12):741-756. doi: 10.1042/CS20231198. Clin Sci (Lond). 2024. PMID: 38895777 Free PMC article. Review.
Cited by
- Type 2 diabetes mellitus related sarcopenia: a type of muscle loss distinct from sarcopenia and disuse muscle atrophy.
Liu Z, Guo Y, Zheng C. Liu Z, et al. Front Endocrinol (Lausanne). 2024 May 24;15:1375610. doi: 10.3389/fendo.2024.1375610. eCollection 2024. Front Endocrinol (Lausanne). 2024. PMID: 38854688 Free PMC article. Review. - Fine-needle percutaneous muscle microbiopsy technique as a feasible tool to address histological analysis in young children with cerebral palsy and age-matched typically developing children.
Deschrevel J, Maes K, Andries A, Beukelaer N, Corvelyn M, Costamagna D, Campenhout AV, Wachter E, Desloovere K, Agten A, Vandenabeele F, Nijs S, Gayan-Ramirez G. Deschrevel J, et al. PLoS One. 2023 Nov 22;18(11):e0294395. doi: 10.1371/journal.pone.0294395. eCollection 2023. PLoS One. 2023. PMID: 37992082 Free PMC article. - Fortetropin supplementation prevents the rise in circulating myostatin but not disuse-induced muscle atrophy in young men with limb immobilization: A randomized controlled trial.
Lim C, McKendry J, Giacomin T, Mcleod JC, Ng SY, Currier BS, Coletta G, Phillips SM. Lim C, et al. PLoS One. 2023 May 23;18(5):e0286222. doi: 10.1371/journal.pone.0286222. eCollection 2023. PLoS One. 2023. PMID: 37220119 Free PMC article. Clinical Trial. - Myonuclear permanence in skeletal muscle memory: a systematic review and meta-analysis of human and animal studies.
Rahmati M, McCarthy JJ, Malakoutinia F. Rahmati M, et al. J Cachexia Sarcopenia Muscle. 2022 Oct;13(5):2276-2297. doi: 10.1002/jcsm.13043. Epub 2022 Aug 12. J Cachexia Sarcopenia Muscle. 2022. PMID: 35961635 Free PMC article. Review. - The effect of passive mobilization associated with blood flow restriction and combined with electrical stimulation on cardiorespiratory safety, neuromuscular adaptations, physical function, and quality of life in comatose patients in an ICU: a randomized controlled clinical trial.
de Campos Biazon TMP, Libardi CA, Junior JCB, Caruso FR, da Silva Destro TR, Molina NG, Borghi-Silva A, Mendes RG. de Campos Biazon TMP, et al. Trials. 2021 Dec 30;22(1):969. doi: 10.1186/s13063-021-05916-z. Trials. 2021. PMID: 34969405 Free PMC article. Clinical Trial.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous