An in vivo recombinant RNA capable of autocatalytic synthesis by Q beta replicase - PubMed (original) (raw)
. 1988 Jun 2;333(6172):473-5.
doi: 10.1038/333473a0.
Affiliations
- PMID: 2453805
- DOI: 10.1038/333473a0
An in vivo recombinant RNA capable of autocatalytic synthesis by Q beta replicase
A V Munishkin et al. Nature. 1988.
Abstract
A variety of small RNAs ranging from tens to hundreds of nucleotides in length grow autocatalytically in a Q beta replicase (Q beta phage RNA-dependent RNA polymerase) reaction in the absence of added template, and similar RNAs are found in Q beta phage-infected Escherichia coli cells. Three such RNAs have been sequenced. One of them that is 221 nucleotides (nt) long ('MDV-1' RNA) has been found to be partially homologous to Q beta phage RNA 8, which might be considered as an indication of its origination from by-products of the Q beta RNA replication. To gain further insight into the origin and function of these RNAs, we have sequenced a new RNA, 120 nt long, isolated from the products of spontaneous synthesis by the nominally RNA-free Q beta replicase preparation. The minus strand of this RNA appeared to be a recombinant RNA, composed of the internal fragment of Q beta RNA (approximately 80 nt long) and the 33-nt-long 3'-terminal fragment of E. coli tRNA(1Asp). This seems to be the first strong indication of RNA recombination in bacterial cells. The various implications of this finding are discussed.
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